FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n01_mb88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n01_mb88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25084539
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG303480.12098288910152982No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG34600.032.015171
TATGCCG23300.027.24248949
TTGACGG38650.026.42745231
CTACGGT29300.026.02013613
TACGGTG29350.025.97580714
ATCTCGT42250.025.31146669
CCGTCTT25750.025.18636753
TCCGCAA31750.024.23596633
GACGCAC34000.023.6411636
TGCCGTC27650.023.45560851
ACGGTGA33900.022.69299515
CCTACGG33900.022.59091612
TGACGGC45750.022.47672532
GTCCGCA35200.021.95903432
ACGTAGA528300.021.70514769
ACGCACC35950.021.5905747
GCCGTCT29100.021.57557952
GACGGCT46750.020.88834433
TAGACGC66950.020.1474344
ATGCCGT33900.019.94537250
Produced by FastQC (version 0.11.2)