FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n01_mb87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n01_mb87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31926030
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG353220.11063699432719946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG43550.039.05266649
CCGTCTT43150.039.0143953
TGCCGTC46200.037.63359551
GCCGTCT46050.037.1568252
CGTCTAG40350.033.2679371
ATGCCGT61100.028.28701450
CTACGGT32800.025.55745313
TACGGTG33650.025.11694514
TTGACGG49100.024.73843231
ATCTCGT75100.024.5294569
GACGCAC38400.022.9982036
TGACGGC53800.022.76911732
CCTACGG37950.022.54351612
TCCGCAA36650.021.93689733
GACGGCT53500.021.477533
ACGCACC41500.021.2800647
ACGGTGA40200.021.02457415
CTAGACG41900.020.9948443
GTCCGCA40400.020.41345832
AACTGCG39150.020.2664247
Produced by FastQC (version 0.11.2)