FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n01_mb86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n01_mb86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27374545
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT40700.043.90509453
TATGCCG42400.042.79594449
GCCGTCT43600.041.38010852
TGCCGTC43950.041.2860451
CGTCTAG34250.034.05451
ATGCCGT56550.032.2703950
TACGGTG29550.028.13444114
CTACGGT29300.028.02128813
TTGACGG41000.027.01764731
GACGCAC33750.025.450976
TCCGCAA32950.024.71550233
CCTACGG33750.024.63281612
ATCTCGT56000.024.02455543
ACGGTGA35900.023.83065615
TGACGGC47000.023.78859132
CTCGTAT71450.023.41647145
CGTCTTC77750.022.89433754
GTCCGCA36000.022.7178632
ACGCACC36900.022.716997
GACGGCT46900.022.51445433
Produced by FastQC (version 0.11.2)