FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n01_mb85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n01_mb85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33032219
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG363200.10995325503260922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC51450.041.43697451
CCGTCTT52250.040.6045253
TATGCCG53050.040.18740549
GCCGTCT54500.038.92806652
ATGCCGT65050.032.8799150
CGTCTAG37100.030.6951831
CTCGTAT78950.026.2171445
TTGACGG43350.025.15377631
CTACGGT34500.024.79812213
CGTCTTC88050.024.13442254
TACGGTG36450.023.5661514
TCTCGTA83350.023.42597844
ATCTCGT83350.023.21896469
TCCGCAA36000.022.81213433
TGACGGC49150.022.25531432
ACGCTAC817850.022.06884669
GACGCAC40450.021.9182266
CCTACGG39500.021.6588612
ACGCACC41750.020.7394647
GTCCGCA39650.020.71246332
Produced by FastQC (version 0.11.2)