FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n01_mb84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n01_mb84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29332468
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG300950.10259961759781006No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG40000.031.315571
ATCTCGT48650.027.3705969
CTACGGT32450.026.68347513
TTGACGG44650.026.5098431
TACGGTG33350.025.5496714
TGACGGC49250.024.24317632
GACGCAC39100.022.2333536
CCTACGG39750.022.12976612
TCCGCAA38350.022.04495833
CTAGACG40750.022.0104963
GACGGCT53350.021.53864333
ACGGTGA40250.021.51266315
ACGCACC40550.021.267787
GATCTCG70550.021.17242469
GTCCGCA40500.020.70479232
TAGACGC73950.019.8259564
CGGCTAT65550.019.63558835
GGTACGA32950.019.37084618
CGCACCT45750.018.6996578
TGCGACC33800.017.96259110
Produced by FastQC (version 0.11.2)