FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n01_mb83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n01_mb83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28922468
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG317450.10975895971256672No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG31750.036.39892649
TGCCGTC33200.035.3285751
CCGTCTT33200.034.70506353
CGTCTAG38950.033.0465051
GCCGTCT35650.032.61030652
TTGACGG45650.026.07883331
ATGCCGT48400.024.16253950
TGACGGC49600.024.00157532
CTACGGT34900.023.22876513
GACGGCT51050.022.77905733
TACGGTG36000.022.71076814
TCCGCAA35050.022.4464933
GACGCAC38700.021.2155826
ATCTCGT47750.021.16801869
GTCCGCA37150.021.08479732
ACGGTGA39900.020.83682815
CCTACGG39750.020.65474112
GGTACGA40450.019.95841818
ACGCACC41250.019.5691457
AACTGCG38900.019.3324477
Produced by FastQC (version 0.11.2)