FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n01_mb82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n01_mb82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28268940
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC30100.036.44546551
CCGTCTT33600.032.95718453
CGTCTAG33400.032.648851
GCCGTCT33800.032.4559352
TATGCCG33450.032.38305749
CTACGGT29600.025.2902513
TTGACGG39950.024.79037731
TACGGTG31000.024.59336714
ATGCCGT45750.024.20462250
GACGCAC34200.022.5948456
TGACGGC43450.022.3959232
ATCTCGT51100.022.2780669
GACGGCT42050.022.23840533
TCCGCAA32650.022.19404433
CCTACGG34550.021.76647812
ACGGTGA35850.021.45880315
GTCCGCA35350.020.88978232
ACGCACC36700.020.7733237
CTAGACG38200.020.3193783
TAGACGC69700.018.5109444
Produced by FastQC (version 0.11.2)