FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n01_mb81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n01_mb81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26160718
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG277790.10618592349032623No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT26750.039.72018453
TGCCGTC29200.036.97820351
TATGCCG29350.036.78914649
GCCGTCT30600.034.60984452
CGTCTAG31400.032.9706761
TTGACGG38950.025.86922531
CTACGGT28050.024.59691213
AGACGGC54200.024.50440269
TACGGTG28950.024.07056414
ATGCCGT45700.023.92911150
ATCTCGT45800.023.3495769
TGACGGC43050.023.32506432
GACGCAC33800.022.4539956
GACGGCT42900.021.95836833
ACGGACT425850.021.5156369
ACGCACC34150.021.3142597
CCTACGG33100.021.05241412
TCCGCAA33200.020.68281733
ACGGTGA35750.019.87816615
GTCCGCA34650.019.6185132
Produced by FastQC (version 0.11.2)