FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n01_mb80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n01_mb80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31927243
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG359540.11261229164071575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG45100.044.13518549
TGCCGTC47400.041.62938351
CCGTCTT47550.039.4668753
GCCGTCT48400.039.0586752
CGTCTAG41850.035.2095871
ATGCCGT64300.030.63445350
ATCTCGT53250.028.11614643
CTACGGT35150.027.48014813
TACGGTG35100.027.42105114
TTGACGG50550.027.23690831
GACGCAC38850.024.5965636
CCTACGG39250.024.25766412
ACGGTGA40300.024.22533415
TCCGCAA38250.023.99628633
TGACGGC57800.023.34265332
GACGGCT56600.023.10556433
ACGCACC41350.022.6920097
GTCCGCA40650.022.66480632
CTAGACG44050.022.2411943
CGTCTTC88200.021.042554
Produced by FastQC (version 0.11.2)