FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n01_mb79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n01_mb79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39249741
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG402260.10248730048944782No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG67150.047.5203749
CCGTCTT66350.047.10535453
GCCGTCT69350.045.5151552
TGCCGTC71050.044.7660551
ATGCCGT90200.035.56813450
CGTCTAG50850.034.813991
ATCTCGT92650.029.48892443
CTCGTAT105200.028.59465645
TTGACGG59950.027.11122931
TCTCGTA109550.026.79789744
CTACGGT42350.026.55522513
CGTCTTC118350.026.43756954
TACGGTG45200.024.80457714
TGACGGC68850.024.00700432
GACGCAC51000.022.7953976
GTATGCC142450.022.73983248
CCTACGG50150.022.69971812
TCCGCAA48950.022.20534933
ACGGTGA51650.022.17468315
CTAGACG53400.022.1586673
Produced by FastQC (version 0.11.2)