FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n01_mb78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n01_mb78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39365059
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG51450.031.7924731
CTACGGT41300.026.2278413
TTGACGG60750.025.10641531
TACGGTG44150.024.69112814
TCCGCAA45350.023.43500333
ATCTCGT69600.023.39457369
TGACGGC68600.022.23302732
CCTACGG50300.021.8776212
GATCTCG104400.021.5111169
TATGCCG33200.021.30107149
GACGCAC52200.021.2800256
CTAGACG52950.021.2393473
GTCCGCA50250.020.94422132
ACGGTGA52900.020.80282615
ACCGTAC708950.020.79216269
GACGGCT70900.020.6353233
TAGACGC95500.020.5179484
ACGCACC54350.020.0569257
TGCGACC43050.018.91107610
GACCGTA981850.018.87791669
Produced by FastQC (version 0.11.2)