FastQCFastQC Report
Mon 9 Apr 2018
HLVVFBGX5_n01_mb77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLVVFBGX5_n01_mb77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36983277
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG403720.10916285217234807No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41250.034.0374949
TGCCGTC41500.032.5022551
CGTCTAG50050.031.9243011
GCCGTCT42000.031.78672852
CCGTCTT42150.031.42811853
TTGACGG60650.024.80623431
CTACGGT41200.023.52857413
TACGGTG41500.023.19229914
TGACGGC65350.023.02147332
ATGCCGT60750.022.94151750
GACGCAC46850.020.9859036
TCCGCAA45150.020.78724133
GACGGCT69100.020.77308533
ACGGTGA48500.020.34296815
GGTACGA49450.020.2330918
CCGTACG44600.020.1925633
CCTACGG49050.020.04409812
ATCTCGT53900.019.84075743
ACGCACC49450.019.7424377
GTCCGCA49850.019.58905432
Produced by FastQC (version 0.11.2)