FastQCFastQC Report
Fri 28 Jul 2023
HLTLYDMXY_n02_CIVR__Ferret_mRNA_P3_H12_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n02_CIVR__Ferret_mRNA_P3_H12_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62076
Sequences flagged as poor quality0
Sequence length151
%GC81

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23793.832398994780591No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGG3690.5944326309684902No Hit
ATCACCGACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC2590.4172304916553901Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGG2450.39467749210645015No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGG1670.2690250660480701No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT1350.2174753527933501No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG1280.20619885301888008No Hit
GATCGGAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCACACAAC1270.20458792447967006Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCACACAACGTGTAGATC1090.1755912107738901Illumina Single End PCR Primer 1 (96% over 33bp)
CTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCACACAAC1050.16914749661705006Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCCGGGGGGGGG1050.16914749661705006No Hit
ATATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCCTCGATG1010.16270378246021006No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG1010.16270378246021006No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGG980.15787099684258007No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCCGGGGGGGGGG950.15303821122495007No Hit
GCCTTAATATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCC920.14820542560732006No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGG800.12887428313680005No Hit
CCTTAATATCTCTGTTGCACGCCGGAGTTCATAAGAGATATTAAGGCCCT760.12243056897996005No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGCGGGGGGGGG700.11276499774470004No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGTGG630.10148849797023003No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG630.10148849797023003No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)