FastQCFastQC Report
Thu 20 Jul 2023
HLTLYDMXY_n02_CIVR__Ferret_mRNA_P3_B3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n02_CIVR__Ferret_mRNA_P3_B3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCCAAGACATCAACAACGATCTACTATAACAAAACTCTAACCTGAG14.0No Hit
CTACAAACGAGGCAATACAGCCACACCGCCGTGAAGCAGGACATCCAAAC14.0No Hit
CACCCCTAAACCGTACACTACTTATACAACATACACAACAGACACACAAA14.0No Hit
ATCCATTCCATGTGTGATGCTCATTGGGTTGTCTGCTATACTCTCTGCTG14.0No Hit
CTAAAACTCACACATACTGCAAATCAAAAAAAAACAAAAAACAAAAAAAA14.0No Hit
TGGACACAACATGATCAGTATTATAGACGTTGAGAGAAATCTGAAGTCCA14.0No Hit
CCTGGAAGAGCTTGAAGATACGTGGCTTCCTTATCTGACCCCCAAGGATG14.0No Hit
CGCAAACAGGAAAAATTAGAAACGCACCTCAAAAACAAATTCAACACTGA14.0No Hit
CAGTAGGTAGAGCCTTACCGGCATGTAGCTGATAATGATCGTCTGGATGG14.0No Hit
CATCCCCACAATCAGTTCTCAGAACAACATGCATGCCCGCAACCTGGACA14.0No Hit
CATCGATGCCTTGGAGTTCTACCCAGGGCTGCTTCTGGAGAAGTGCCATC14.0No Hit
CAGAAAGTATTTCATGAAATAGAAAAAGCAAGAATAAAAGTAAAAAAACA14.0No Hit
ATTCATATAAAAAATCAAAAGATATTATGCAATATCAAAAAAAAACAAAA14.0No Hit
ATGAATTTGCCAGATAAAGAGACCTCAAGAAAAGACAATAACCATTATTC14.0No Hit
CAAATGAAGGACGTTCGTGCAATCTTGCAGCATAGCAAGAGCTCCTGCCC14.0No Hit
CCAACCGCGACCCAGGCACGTCTTACACAGGCGCCGCCCAGCAGCAAAGA14.0No Hit
TTTTCCTTTCCTACTTGGCGAGATTTGGCTTTCCGTTCCAGGATCTTTTT14.0No Hit
CAAACAGCAATAAAACTACTTTGAAGACATAAGATCTCAGCGCAACCAAG14.0No Hit
GCCATTATAAAATGGATCATGTATATTATTACAACGTATTAATAAAATAA14.0No Hit
TTGGAATCAAGCACAGGTGGGCAGATTTGTTTTGATAGGTGAGGTCTGAG14.0No Hit
CTTGATCACAATACTCATCATACTATAAATCAGTCTCACTCAATGAACAC14.0No Hit
CTCCTCGCTGTACGGGTAGCTGGGAGGGAACGAGATGTCCAGCTCATACA14.0No Hit
GGCGCCTCTGACACCTTACGAGGCACAGCCTCTCGACTGTTCTGCACTCA14.0No Hit
GAAAGTTTGAACTCACAGGCATACCTCCTGCTCCTCGTGGTGTCCCTCAG14.0No Hit
GCACATTTCCACTACGTCCTTTCAATAGGGGCAGTATTTGCACTTATAGG14.0No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)