FastQCFastQC Report
Thu 20 Jul 2023
HLTLYDMXY_n02_CIVR__Ferret_mRNA_P3_A8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n02_CIVR__Ferret_mRNA_P3_A8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGAACCCACATCTTCTGCAAAGAACACCAGTTTTTGATGATGAACTTC30.2066115702479339No Hit
TCTGCTGCCTCATTGTTAGTGTATAGAAATGCAACTGACTTCTGTGCACT30.2066115702479339No Hit
CGCCGTGTTCCCAGCCGCGTCCACTCAGCTCCCGTCCACACGTCCCCCTC30.2066115702479339No Hit
CCTTCCGGCCCTCCTGCCCCGCGAAGGTGAAGAAGGTGGTGACCACCGTC20.13774104683195593No Hit
GCTGGTGTCTGTTTTCGATGCAAGAGAACTGGAACTGGTCATCGCAGGCA20.13774104683195593No Hit
TCTAAATTGTGGAATAATGTTGAGAGAATGCATCAGACATGAACCACTTG20.13774104683195593No Hit
GGATTCTCAGTAGATAAAGCTACCCTAACACGATTCTTCGCTTTCCACTT20.13774104683195593No Hit
GCCGGTGCTGAAGCAGCTGGGCCCCGCGCAGCCCAAGAAGCGGCCTGAGC20.13774104683195593No Hit
TTGATTTGTAAATTCTTTATTTTGGCTAATATTGACTGCCTTCATTACTA20.13774104683195593No Hit
CAGCTCTGCCACTTCCCTGCTGAGTGCTCTTGGACAAGTTACTGAACATC20.13774104683195593No Hit
TCTGGCAGCAGCCACTGCAGCGGCAGCACGGTGGGCAGCCGCACAGCAGG20.13774104683195593No Hit
GGCCACTGCCCGTGCGGCTGGGATTTCTGACGCCCCGTCTGGCTTGCGAA20.13774104683195593No Hit
CTCTGCGACGTCGGGCACTGGGACCCGGAGCTGCGCGGCGGGCAGGGGCG20.13774104683195593No Hit
GTGACCAGGAGCTGGGTGAACTGGGGGCTCAGGTTGTAGCCCATCTGGGA20.13774104683195593No Hit
TGGAAGTCCTGGGACTCAGGCCCCTCTCTGGCCGAGGGTGGGACCCGCTG20.13774104683195593No Hit
GGCAGCTTCTGCATTGGGCGGTTCATGCACCCAGGGTGGGCGTGGGGGAG20.13774104683195593No Hit
CTGGAATTGCTACAGGAGCTGAACAGGACCTGCCGAGCCATGCAACAGAT20.13774104683195593No Hit
GAAACTCTTTGTGGCAGATAATTGGGTTTTGACACCGTGAAAAGGCCCAG20.13774104683195593No Hit
GTTCACTGGATCTGAACAACCCTGCTGGAAACAAAAAGCAATACTCTTCC20.13774104683195593No Hit
CCATGACCCTTCCCGCTAGCTGCCTTCGTGTTGGGCCCTGTCTTTGCCGA20.13774104683195593No Hit
CGGCTGTCAAAAGGGGCAGTCTGGTAGTTCTTGATTTTGGTCTTGATGTC20.13774104683195593No Hit
GCGCGCAACATCCAACGTATTTGCCATGTTTGATCAGTCACAGATTCAGG20.13774104683195593No Hit
CTCAGGAAGGCGTCAGAGTGCCACGGTCCTGAGTAGACTCCTCGGTGCGT20.13774104683195593No Hit
GAAATGCCATCTGGCAACATGGGGACAAGGGGGTGGCCTGAGATGGTTTC20.13774104683195593No Hit
CTTGCACGATTGGTTAAGGAAAAATGGATTATTCCCTAATTTCTGAGGCA20.13774104683195593No Hit
GGAGAACTGAACTGTGGGCGTTTGATGCCCTTGTTTGGGAGGAGCAGACG20.13774104683195593No Hit
TGTCTGTCTCGCTCGCAAACCATAGTCACAACACCTAAAACAATACCTAC20.13774104683195593No Hit
ACAGCATTCAGGGGTGAGGGACCTCCAGGCCGCTTGGGAGGCCCCTCTGC20.13774104683195593No Hit
GGCGGTTCTTGTTAAGCTCCCCTATGCCACCCAGCAGGAAGCCAGTCACC20.13774104683195593No Hit
AAGATATTTAGACAAACATCTAACATTGATTAAACCTTTGAAAACATCAT20.13774104683195593No Hit
CCGCTCACTGGGTTGATCCGCTGGTGCGTGAAGGCCCCCCTGGCTTACAA20.13774104683195593No Hit
CCCAGGGCATACCCCTGCACCTTGCCCCCAGCCCTGTGCTGCTTCTCAGT20.13774104683195593No Hit
CCCTAGTTCTCCTTCTCCTGCACAGTCTTGGTTCCCCGGAGGCGCCCAGT20.13774104683195593No Hit
GGCGCACCTGACATAGCATTCCCACGGATAAACAACATAAGCTTCTGGCT20.13774104683195593No Hit
ACCACTGCAGCCCTCCCAGGATAACGAGCTACAGAACCAGTACCTGGAAT20.13774104683195593No Hit
GGCCAAGCCTGTGGCCTCTGCTTTGAGTGCTGCAGAGGCTGGTACAGAGG20.13774104683195593No Hit
TGACCACTAGTTGATGGCCTGCTGCTGGCTGCAAAGTTTTTGAGGGTTTT20.13774104683195593No Hit
TGAATATGGACTCTGGAGACTCCGAAGTGGTCGATGGACTTATTTTCCAG20.13774104683195593No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCAC20.13774104683195593No Hit
CAAAATTGCTTGCAAAAGTGGCACAGAGTTAGCACTCCATACCCCTTCAA20.13774104683195593No Hit
CTGACAGGAGCTCTTTCCGCCCTACTTACAACATCAGGACTAGCAATATG20.13774104683195593No Hit
CGCTGCTCGAGCTGCAGTTGGAGGGCGAGCGGCAGCTGCACAAGCAGCCG20.13774104683195593No Hit
CAGAGGGTTTTTCCTTACATAACACTTTGTCTATTGAAGAAAGATGTCAT20.13774104683195593No Hit
CAGCATTCTCTTAGATCTGCAATTGGGCTCAACACATTCAAGCCTCAGCA20.13774104683195593No Hit
CCCACACCGGCCTTTCCCCCGGGGCCCTACGCTACGCCTCCGGGGTACGG20.13774104683195593No Hit
CTCAGCTACCCCTCTCAGGTGTTGGGGTTACTTCTGTAGGATGGATTTCA20.13774104683195593No Hit
CCTAGCCTGGGCAGGCCGCCTCTTCCTGTCCCCACTGGCTGGGGTACAGG20.13774104683195593No Hit
GTTTGGGCTGTAGGAATCAACTGAGAGGTGTCCCTTTCCTGAAGCTACAT20.13774104683195593No Hit
TGACGAATACCTGAGCAGCCTTCTTACTCAGCTCAACGGCAATGTCTGAG20.13774104683195593No Hit
GTTTTGGAGATACTGAATGATAACATTTTATAAGCACCGGTCATTTTTTG20.13774104683195593No Hit
GGCCTGGCAGGGTGCCTGTCCCCCGGGCCCTGGGGCTGATCTCAGGGAAG20.13774104683195593No Hit
CGAAGAGAATCCGGCGGGAATCGGAGCCATCAGAACCGCCACCATGACCG20.13774104683195593No Hit
GGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATT20.13774104683195593No Hit
AGAAAATGCTGCAGAATTTTTGAAGCAACATAATTCAGGTCCCAAGGCCA20.13774104683195593No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT20.13774104683195593No Hit
CTCATGTTTATGTACTCACTGGCTGTCCTCCGAGGGCGCTGCCTAAACTT20.13774104683195593No Hit
GTCCCAGAGCTGGGTCCTCACCCTCTGCACACCTGCTCCATCCCAGGGCC20.13774104683195593No Hit
TCATATTAAAACTATTAAATAAGATATATTCAGAATAGTACAGCTTTCAT20.13774104683195593No Hit
CTGGGCTGCGGAGGGTCTCTGGCTGGGTGAACTGTTTCTGCAGGAAAGGG20.13774104683195593No Hit
CTCAGCTAAAGGTGTCCGATGCTGTCTCAGCTGACGGATCATCTCCTGTG20.13774104683195593No Hit
GTTTCCCCAGAAGGGTTTTTAAACCATATCACAAACCTGGAAAGTATTAA20.13774104683195593No Hit
CTCTACACTTAGCTGGTGTTTCATCTATCTTAGGGTCAATCAACTTTATT20.13774104683195593No Hit
TGGGAGTTGGTGTGCAAGAAGGCTAAAACAACAGAACTATAAAACAGTGT20.13774104683195593No Hit
CGTCGCCGGCTGCTGCGCCAGCAAGCACAGGCTGGGGAGACGGCAGCAGC20.13774104683195593No Hit
CTCAACGCTTGAAACATAGCTAGTGCTAACTGAGATGTGCCGCGCACGTG20.13774104683195593No Hit
CTGTGTCAGTACCATAGTCTGAAAAACAGACAGGTGATCTTTAATGAACT20.13774104683195593No Hit
CGACCACAAGGACTATCACCACCACTATGATGAGAAGGCGCTTGAGACTT20.13774104683195593No Hit
GGTTTCTTCTGCAGCTCTGACCCCCAGCCCAAGGCTCTTAGACCTGATAA20.13774104683195593No Hit
AAATAAATAGTCAATGTCGTTTAGCTAATAACATTTCAGAGTCCCTGGCC20.13774104683195593No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)