FastQCFastQC Report
Thu 20 Jul 2023
HLTLYDMXY_n02_CIVR__Ferret_mRNA_P2_D6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n02_CIVR__Ferret_mRNA_P2_D6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196823
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25191.279830101156877No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG4470.2271076042942136No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTGCTAGTGTAGATC3720.18900230156028514Illumina Single End PCR Primer 1 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT3410.1732521097635947No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT3310.16817140273240425No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG3180.16156648359185666No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG2570.13057417070159483No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG2130.1082190597643568No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)