FastQCFastQC Report
Thu 20 Jul 2023
HLTLYDMXY_n02_CIVR__Ferret_mRNA_P1_H7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n02_CIVR__Ferret_mRNA_P1_H7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186007
Sequences flagged as poor quality0
Sequence length151
%GC80

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGGAGTAGTGTAGATC66553.5778223400194618Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61433.302563882004441No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG8920.47955184482304425No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT7100.38170606482551733No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG6790.3650400253753891No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT6650.35751342691404087No Hit
CTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGGAGTA6310.3392345449364809Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGGAGTA6280.33762170240904915Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG4990.2682694737294833No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG4650.2499905917519233No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGTGT4300.23117409559855273No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTG3830.2059062293354551No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG3650.19622917417086455No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTG2980.16020902439155515No Hit
ATCACCGACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC2940.1580585676883128Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTGT2870.1542952684576387No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG2760.14838151252372223No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTG2670.14354298494142695No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGTGG2600.1397796857107528No Hit
GATCGGAAGAGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGGA2600.1397796857107528Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGT2530.13601638648007872No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTGG2520.1354787723042681No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGT2430.13064024472197283No Hit
GCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGGA2410.12956501637035167Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGG2310.12418887461224577No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTT2260.12150080373319284No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGT2170.11666227615089754No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTT2080.11182374856860226No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGT1930.10375953593144344No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGG1930.10375953593144344No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGT1900.10214669340401167No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGT1900.10214669340401167No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)