FastQCFastQC Report
Fri 28 Jul 2023
HLTLYDMXY_n02_CIVR__Ferret_mRNA_P1_F2_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n02_CIVR__Ferret_mRNA_P1_F2_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11326443
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCG205320.18127491569948306No Hit
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG203610.17976517429169953No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC183470.16198377548891563No Hit
GGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACA172770.1525368555688666No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC161360.14246308395318813No Hit
CGGAGAGGCTCTGGAGAGAACCTTCGCGTCCTTCCCCACCACCAAGACCT150930.1332545442554207No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC148210.1308530842383615No Hit
GGGAGGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGA127960.11297456756724067No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC127900.1129215941845114No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA120790.10664424833109565No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA119010.10507270464346133No Hit
CGGAGAGGCTCTGGAGAGGACCTTCGCGTCCTTCCCCACCACCAAGACCT116450.10281250698034679No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCCCACATGGACG114590.10117033211574013No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)