FastQCFastQC Report
Fri 28 Jul 2023
HLTLYDMXY_n02_CIVR__Ferret_mRNA_P1_B5_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n02_CIVR__Ferret_mRNA_P1_B5_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11939556
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG793110.6642709326879492No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC692660.5801388259328907No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC461070.3861701389900931No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA444900.37262692180513246No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC420380.35209014472564976No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC384160.3217540082730045No Hit
CGGAGAGGCTCTGGAGAGAACCTTCGCGTCCTTCCCCACCACCAAGACCT384110.3217121306688457No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA340900.28552150515479807No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT334440.28011091869747917No Hit
GGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCG331380.27754800932295975No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA296200.24808292703681778No Hit
GGGAGGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGA276270.23139051401911429No Hit
CGGAGAGGCTCTGGAGAGGACCTTCGCGTCCTTCCCCACCACCAAGACCT270090.2262144421450848No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC256250.2146227213139249No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC235730.1974361525671474No Hit
GGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACA231780.19412782183860103No Hit
CTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACCT220230.18445409527791484No Hit
GGACAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTAGACGA218740.18320614267398216No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT217750.18237696611163765No Hit
ACACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGA215500.180492473924491No Hit
GAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCA206990.17336490569666074No Hit
GGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCACGCCTACA206120.17263623538429737No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC205150.17182380986361637No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA203100.17010682809310498No Hit
GTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAG201530.1687918713225182No Hit
GGGTCTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTG193460.16203282601128552No Hit
GTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCC192940.16159729892803384No Hit
CAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCAC181770.15224184215895464No Hit
GACACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTG179430.15028197028432214No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCCCACATGGACG178200.1492517812220153No Hit
GTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTC175760.14720815413906513No Hit
CCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCA173260.14511427393112442No Hit
GTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAG172760.14469549788953626No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT168620.1412280322651864No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA165470.13858974320318107No Hit
CGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGA163360.1368225083076791No Hit
CCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGG160150.1341339661206832No Hit
CCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACC160100.1340920885165244No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG155800.13049061455886635No Hit
TGGCAGGCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGAC154830.12967818903818534No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG153980.1289662697674855No Hit
GGACGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCT153390.1284721140384115No Hit
GCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGG152110.12740004737194582No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA151930.1272492879969741No Hit
GCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGG149190.12495439528907104No Hit
CGGCGGGGGTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGG148900.12471150518494993No Hit
GCAGCCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCAC148550.12441836195583823No Hit
GTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCT147540.12357243435183016No Hit
GAAGAATCTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGC147340.12340492393519492No Hit
GGAGAGGCTCTGGAGAGAACCTTCGCGTCCTTCCCCACCACCAAGACCTA146690.12286051508113031No Hit
AGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCA138980.11640298851984111No Hit
GGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTA138180.11573294685330007No Hit
GCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTT137140.11486189268679672No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT135260.1132872947704253No Hit
CGACCAGCAGCCTGCCCAGGGTCTCACCACCAACTTCGTCCACGTTCACC135170.11321191508293943No Hit
GGGAAACAGGGTCTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGT134120.11233248539560432No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA133660.11194721143734322No Hit
CGGCGCTGAAGAACTTGTCCAGGGAGGCGTGGACGGCGGGGGTGAATTCC132480.1109588999791952No Hit
GGCAGGCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACG131800.11038936456263533No Hit
GGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAG131220.10990358435439308No Hit
GCTGAAGGTTCTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTA128510.10763381820898532No Hit
GTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGAGCACC127740.10698890310493958No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA126390.10585820779265159No Hit
CCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGG125390.10502065570947529No Hit
CGGGAGGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCG125100.10477776560535418No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG124600.10435898956376603No Hit
CTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGA124360.10415797706380371No Hit
GGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAAGGTTCTCTCCAGAG123900.10377270310554262No Hit
TCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCC123030.10304403279317925No Hit
CTGGAGAGGACCTTCGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCA122960.10298540414735691No Hit
GCAACCCCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGC122720.10278439164739459No Hit
GCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACC122610.10269226091824521No Hit
GCGGGGGTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCA122200.10234886456414291No Hit
CGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCACTTCGACCTGAGCC120730.10111766300187376No Hit
CTGGTGTGCGTGCTGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGT120340.10079101768943502No Hit
GGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAAGGTTCTCTCCAGA120170.10064863383529504No Hit
GGCCAATGCCCTGGCTCACAAGTACCACTGAGACCCTGGCCTATTTCCTG119860.1003889926895104No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)