FastQCFastQC Report
Thu 20 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P3_H5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P3_H5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103096
Sequences flagged as poor quality0
Sequence length151
%GC64

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAGCGAATCTCGGGT85128.256382400869093TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAGCGAATCGCGGGT58095.634554201908901TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAGCGAATCTCGGTT36553.545239388531078TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAGCGAATCTCGGGG22442.1766120897028016TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAGCGAATCGCGGGG18821.8254830449289983TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAGCGAATCGCGGTT15571.5102428804221308TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAGCGAATCTCGGTG8580.8322340342981299TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAGCGAATCGCGGTG4690.45491580662683323TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAGCGAATCTCGTGT3610.35015907503685884TruSeq Adapter, Index 22 (97% over 37bp)
ATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2230.21630325133855824Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAGCGAATCTCGTTT2100.20369364475828355TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAGCGAATCGCGTGT1710.16586482501745944TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAGCGACTCGCGGGT1160.11251648948552805TruSeq Adapter, Index 22 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAGCGAATCTCGGG1120.10863661053775121TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAGCGAATCTGGGGT1080.1047567315899744TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)