FastQCFastQC Report
Fri 28 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P3_H12_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P3_H12_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62076
Sequences flagged as poor quality0
Sequence length151
%GC73

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCCGACATCTCGTAT2366438.12101295186546TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCCGACATCGCGTAT1380222.23403569817643TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCCGACATCTGGTAT2710.4365616341259102TruSeq Adapter, Index 22 (97% over 39bp)
ATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2680.4317288485082802Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCCGACATCTCGTTT2180.3511824215477802TruSeq Adapter, Index 22 (97% over 39bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCCGACATCTCGTA2070.33346220761647016TruSeq Adapter, Index 22 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCCGACATCGCGGAT1570.2529157806559701TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCCGACATCTCGGAT1480.2384174238030801TruSeq Adapter, Index 22 (97% over 39bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCCGACATCGCGTA1420.22875185256782007TruSeq Adapter, Index 22 (97% over 36bp)
CTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCCGA1350.2174753527933501TruSeq Adapter, Index 22 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCCGACATCTCGTATG1120.18042399639152007TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCCGA1070.17236935369547007TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCCGACATCGCGTTT1070.17236935369547007TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCCGACATCGGGTAT1040.16753656807784006TruSeq Adapter, Index 22 (97% over 39bp)
ATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGGCCTTA1010.16270378246021006No Hit
GCCTTAATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGG850.13692892583285007No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCCGACATCGCGTATG770.12404149751917004TruSeq Adapter, Index 22 (97% over 38bp)
CCTTAATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGG750.12081964044075005No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCCGACATCGCGTCT700.11276499774470004TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCCGACCTCGCGTAT660.10632128358786005TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCCGACAGCGCGTAT630.10148849797023003TruSeq Adapter, Index 22 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)