FastQCFastQC Report
Thu 20 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P3_G1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P3_G1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12324954
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC792300.6428421558409062No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC532090.43171763561957305No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC459650.3729425683860565No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA453460.3679202372682283No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC425210.3449992592264442No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG422270.34261385478598944No Hit
GGGAGGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGA421790.3422244009997928No Hit
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG404810.328447473313085No Hit
GGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCG377040.30591594905749747No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC352840.28628098733674784No Hit
CGGAGAGGCTCTGGAGAGAACCTTCGCGTCCTTCCCCACCACCAAGACCT351370.2850882851165205No Hit
GGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCACGCCTACA323080.2621348525925533No Hit
GGGTCTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTG301570.24468245479861428No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCCCACATGGACG287120.23295827310998485No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA278350.2258426278913495No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA262240.21277158519212325No Hit
GTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCC257490.20891761543288517No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC255970.207684345109929No Hit
GAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCA250330.20310826312211794No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA249900.2027593774386501No Hit
CGGAGAGGCTCTGGAGAGGACCTTCGCGTCCTTCCCCACCACCAAGACCT248110.20130703936095826No Hit
CCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGG246610.20008999627909363No Hit
GTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAG243370.197461183222266No Hit
GAAGAATCTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGC242810.19700682047170318No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT236010.19148955850058347No Hit
CCCACATGGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCAC227090.18425220897376168No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG226860.18406559570120912No Hit
CTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACCT225150.1826781665878834No Hit
GTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTC221740.17991142198177779No Hit
GTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCT221500.17971669508867943No Hit
GGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACA217860.17676333721002122No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG215150.1745645460421191No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT210780.1710188938636201No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC210320.17064566731851494No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA208220.16894180700390443No Hit
CGGCGCTGAAGAACTTGTCCAGGGAGGCGTGGACGGCGGGGGTGAATTCC200230.16245902418783875No Hit
CCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACC198860.16134745817306906No Hit
GGACAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTAGACGA196030.15905130355861774No Hit
TGGCAGGCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGAC192390.15609794567995955No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA192290.1560168094745019No Hit
CTCAGGTGCAGGCTGCCTATCAGAAGGTGGTGACCGGTGTGGCCAATGCC190570.15462126674063045No Hit
CGGGAGGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCG189700.15391538175314895No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG189310.15359895055186412No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC188130.15264154332746394No Hit
CCGCCGTCCACGCCTCCCTGGACAAGTTCTTCAGCGCCGTGAGCACCGTG187790.15236568022890795No Hit
CTTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCA186600.151400159383962No Hit
GTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGAGCACC186080.15097825111558227No Hit
CGGCGGGGGTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGG182710.1482439609916597No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA178650.14494983105007936No Hit
GCAGCCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCAC175430.14233724523434327No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT174930.14193156420705505No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA174440.14153399680031262No Hit
CGCTGACCAACGCCGTGGCCCACATGGACGACCTGCCGGGCGCGCTGTCC173780.14099849784429214No Hit
AGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCA172870.1402601583746276No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG169690.13768002704107454No Hit
GTCCATGTGGGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT165650.13440212434058577No Hit
CCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCAC162960.13221956041377517No Hit
CGGGCGAGTATGGCGGAGAGGCTCTGGAGAGAACCTTCGCGTCCTTCCCC159060.1290552484009271No Hit
TCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCC158700.12876315806127958No Hit
GCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTT157890.12810595479707268No Hit
CGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCACTTCGACCTGAGCC154320.12520939226223482No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA153170.12427632589947192No Hit
CGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGAC151110.12260492006704447No Hit
CCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCC150420.12204508024938672No Hit
CCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTAT150280.12193148956174604No Hit
GGGAAACAGGGTCTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGT150010.1217124218070104No Hit
CTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGGCGTT149570.12135542250299677No Hit
CTGGTGTGCGTGCTGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGT148850.12077124182370173No Hit
GGCAGGCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACG148830.1207550145826102No Hit
CCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACT147930.12002478873349141No Hit
CAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGG147700.11983817546093883No Hit
GCGGGGGTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCA145150.11776920222176894No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC144130.11694161292610099No Hit
CTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAG144090.11690915844391793No Hit
GCGACCTGCACGCCTACAAGCTGCGTGTGGACCCCGTCAACTTCAAGCTC142850.11590306949624316No Hit
CACACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGA142260.11542436588404305No Hit
CAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCAC140250.11379352815434442No Hit
CGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGC140210.11376107367216137No Hit
CCGCTCTGAGCGACCTGCACGCCTACAAGCTGCGTGTGGACCCCGTCAAC140200.11375296005161561No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT139960.11355823315851728No Hit
GGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGAGCAC139910.11351766505578843No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG139680.11333105178323585No Hit
CTCAGGTGCAGGCTGCCTATCAGAAGGTGGTGGCCGGTGTGGCCAATGCC138280.11219514490682886No Hit
CCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCA138270.1121870312862831No Hit
GCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGG138090.11204098611645934No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC137430.1115054871604389No Hit
CCCACTCAGACTTTATTCAGAGATCGGGAGGTTGAGGTGCGCGAAGCGCA136770.11096998820441845No Hit
GGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAAGGTTCTCTCCAGAG136200.11050751183330988No Hit
GCTGAAGGTTCTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTA135570.10999635373892674No Hit
CCTTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTC135250.10973671788146228No Hit
GGCCAATGCCCTGGCTCACAAGTACCACTGAGACCCTGGCCTATTTCCTG134560.10917687806380455No Hit
CCACCACCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCC134130.10882799238033666No Hit
CCGGTCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT133850.10860081100505527No Hit
CAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCC133200.1080734256695806No Hit
CGGCAGGTCGTCCATGTGGGCCACGGCGTTGGTCAGCGCGTCGGCCACCT132770.10772453998611271No Hit
GGAGAGGCTCTGGAGAGAACCTTCGCGTCCTTCCCCACCACCAAGACCTA132660.10763529016010934No Hit
GGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCT132230.10728640447664145No Hit
GGACGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCT132040.10713224568627193No Hit
CGACGCGCTGACCAACGCCGTGGCCCACATGGACGACCTGCCGGGCGCGC131610.10678336000280407No Hit
TGAAGGTTCTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATT131320.1065480650069769No Hit
GCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGCT130320.10573670295240048No Hit
CCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCA130150.10559877140312247No Hit
GTCAGCACGGTGCTCACGGCGCTGAAGAACTTGTCCAGGGAGGCGTGGAC129900.10539593088947838No Hit
GTCAAGTCCACCTGGGGCAAGATCGGCGGCCACGCGGGCGAGTATGGCGG129550.10511195417037661No Hit
GGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAG128980.10464947779926805No Hit
CTCTGAGCGACCTGCACGCCTACAAGCTGCGTGTGGACCCCGTCAACTTC128960.10463325055817653No Hit
GGCAGGTCGTCCATGTGGGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT128700.10442229642398666No Hit
CGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCT128650.10438172832125782No Hit
GCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGCTTGA128520.1042762512541629No Hit
CCGGCAGGTCGTCCATGTGGGCCACGGCGTTGGTCAGCGCGTCGGCCACC128150.10397604729396961No Hit
GGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTA128020.10387057022687468No Hit
CACGGCTCCGCCCAGGTCAAGGCCCACGGCAAGAAGGTGGCCGACGCGCT127850.10373263867759668No Hit
TGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTC127730.10363527523104751No Hit
TTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGC127190.10319713972157625No Hit
CTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGA127110.10313223075721013No Hit
CAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTCCACACGCAGCTTGT126930.10298618558738637No Hit
GCGGAATTCACCCCCGCCGTCCACGCCTCCCTGGACAAGTTCTTCAGCGC126590.10271032248883039No Hit
GCAACCCCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGC126140.10234520956427098No Hit
GCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCACGCCTACAAGCTGCGTG125980.10221539163553876No Hit
GCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGG125750.10202877836298618No Hit
TTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCCA125020.10143648406314539No Hit
GCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGG124720.10119307544677245No Hit
GGAGCTTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCG124590.10108759837967753No Hit
GCCCACTCAGACTTTATTCAGAGATCGGGAGGTTGAGGTGCGCGAAGCGC123350.10008150943200274No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)