FastQCFastQC Report
Thu 20 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P3_F9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P3_F9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAGGTAGTCGTACAGGGCGTCGTTCTCGGAGCCCGTCTGCGGGGTGC14.545454545454546No Hit
CCCGTGGATGGCGCGCCTGGGGCCCGGCTGCCCCGACGGGGCCCGGCCGA14.545454545454546No Hit
GCCAGCCAGCCTGATGTGGATGGCTTCCTCGTGGGCGGGGCTTCCCTCAA14.545454545454546No Hit
AACTGCTTCATCGCCGCGGGCCTCTACCTCCTGCTCGGAGGCTTCTCTTT14.545454545454546No Hit
ATTTAAAAAGACCTAAGGCAATGAATTCCATTTCCAATGGAAAAAAAGAA14.545454545454546No Hit
TTTATGAAAACCGAATATACAGCCTTAAAATAGAATGTGGACCTAAATAC14.545454545454546No Hit
CGGCCGCCATCAAGAACGACACAGTAACAATCCGGACCAGGAAGTTCATG14.545454545454546No Hit
TTCCAGCCCTCAAGAGGGGAGGTGGCCCCTTGTGCCTGGGCTTCCCGGGG14.545454545454546No Hit
CGGGAGCCGATGATGATGACCTGCAGCTTGAAGTCGGCCGGCCTGGGGGG14.545454545454546No Hit
GGGAGCTGCAGGAGGCCCCGCTGTCCCTGGCCGAGGGCTGGCAGCTCTTC14.545454545454546No Hit
GTCAGCTTTTGTTTTATGACTTGGGGACTACCAGTCACAGAATCTGCCCA14.545454545454546No Hit
GAAATAATGCTGGTGAAAACTTACCTAAAGTCTTGCCTATCAAATACATC14.545454545454546No Hit
GTCTGTTCTGGGGTTCCCCTCTCATCGGGCCCGTGTCTGCCAGCTGCCCC14.545454545454546No Hit
CGCGACATCATATGCACAGAAGCATAACCTGGAATTCCCTAATCTGACTA14.545454545454546No Hit
CTGATACGAATTTCCGGAAAGTTAACATTTGAACTTAGCTGTTAAGTAAC14.545454545454546No Hit
CTGGAGATGAACAAAAAGCATAATCCCCAACTCAACTTAAAACCTGCACC14.545454545454546No Hit
TGTTACTGGACCTCACCACTACAGCATCTACCCTCATGGATCAACTGATA14.545454545454546No Hit
CGGTCGGGGTGCGGGACCGGCCTGGGAGCTGCAGGACTGCATCACACGGG14.545454545454546No Hit
GCTCCTCAGCAATACAGGGACTCTGCTGCCACTGCTGGATGCGCTGAGGG14.545454545454546No Hit
CAGACGGCCGTGCACGGGAGCCGCAAGCATGATCTGGTCCAGGAGGCCTG14.545454545454546No Hit
GTGCAGCATGAGAGTAGCCTGGTTTTTTGTCTCCTGAGCACTTCCTCCAG14.545454545454546No Hit
CGCAAGATGGTGTTAAAAAGAAGCCCCAGGACAACATAAACCCATCACCT14.545454545454546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)