FastQCFastQC Report
Fri 28 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P3_F11_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P3_F11_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178214
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4285724.048054586059457No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA154708.68057503899806No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCGCAATCTCGGTT41602.334272279394436TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCGCAATCTCGGGT22711.2743106602174914TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCGCAATCGCGGTT18371.030783215684514TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCGCAATCTCGTTT18061.0133883982178729TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCGCAATCGCGGGT17440.9785987632845904TruSeq Adapter, Index 15 (97% over 36bp)
AAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15890.8916246759513844No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10810.6065741187561022No Hit
AAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9420.5285780017282593No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCGCAATCTCGTGT9140.5128665536938737TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCGCAATCTCGGAT6720.37707475282525504TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCGCAATCGCGTTT5820.32657366985758696TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCGCAATCGCGTGT5280.2962730200769861TruSeq Adapter, Index 15 (97% over 36bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4720.26485012400821484No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCGCAATCTCGTAT4520.2536276611265108TruSeq Adapter, Index 15 (97% over 36bp)
AAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4420.24801642968565885No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCGCAATCGCGGAT3600.20200433187067235TruSeq Adapter, Index 15 (97% over 36bp)
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3200.1795594061072643No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3120.1750704209545827No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2540.14252527859764103No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT2440.136914047156789No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT2250.12625270741917022No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2240.125691584275085No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT2180.1223248454105738No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1960.10998013624069938No Hit
AAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1960.10998013624069938No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1950.10941901309661418No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCCGCAATCGCGTAT1820.10212441222350657TruSeq Adapter, Index 15 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)