FastQCFastQC Report
Thu 20 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P3_D6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P3_D6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66380
Sequences flagged as poor quality0
Sequence length151
%GC72

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATGCATCTCGTAT2636539.718288641156974TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATGCATCGCGTAT721310.866224766495932TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATGCATCTCGTTT29494.442603193733053TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATGCATCTCGGAT17562.6453751129858394TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATGCATCGCGGAT9011.357336547152757TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATGCATCGCGTTT5820.8767701114793613TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATGCATCTCGTGT5130.7728231394998494TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATGCATCTCGGTT3120.4700210906899669TruSeq Adapter, Index 3 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATGCATCTCGTA2940.4429044893040073TruSeq Adapter, Index 18 (97% over 35bp)
ATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2840.4278397107562519Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATGCATCGCGTGT2580.388671286532088TruSeq Adapter, Index 3 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATGCATCTCGTATG2080.3133473937933113TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGATGCATCTCGTAT1780.2681530581500452TruSeq Adapter, Index 20 (97% over 37bp)
ATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGGCCTTA1740.26212714673094306No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATGCATCGCGGTT1270.19132268755649293TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATG1260.1898162097017174TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATGCATCTCGTAA1220.18379029828261526TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGATGCATCGCGTAT1060.15968665260620668TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATGCATCTCGTCT1010.152154263332329TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATGCATCTCGTA920.1385959626393492TruSeq Adapter, Index 3 (97% over 37bp)
CTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATG910.13708948478457367TruSeq Adapter, Index 3 (97% over 37bp)
CCTTAATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGG880.13257005122024706No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATGCATCGCGTA840.12654413980114493TruSeq Adapter, Index 18 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATGCATCGCGTCT810.12202470623681831TruSeq Adapter, Index 3 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATGCATCGCGTATG760.11449231696294064TruSeq Adapter, Index 3 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)