FastQCFastQC Report
Fri 28 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P3_C1_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P3_C1_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10211012
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC674180.6602479754210454No Hit
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG464750.45514587584462735No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC450860.4415429146494001No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA406910.3985011475845881No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC375980.36821032038744056No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC353150.3458521055503607No Hit
GGGTCTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTG334610.327695237259539No Hit
GGGAGGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGA318690.31210422629999846No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC316420.30988113616946095No Hit
GAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCA315510.30898994144752745No Hit
GGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCG308410.3020366639467273No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA289380.2833999215748645No Hit
GTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCC276190.27048249478112457No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA273370.26772077047798987No Hit
GAAGAATCTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGC266780.2612669537554162No Hit
CGGAGAGGCTCTGGAGAGAACCTTCGCGTCCTTCCCCACCACCAAGACCT266380.26087521981170914No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG265100.2596216711918466No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCTTATATCTCGTAT249020.24387396665482325TruSeq Adapter, Index 9 (97% over 37bp)
GTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAG241200.23621556805535046No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA237970.23305231645991603No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC230330.22557019813511137No Hit
GGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACA227880.2231708277299057No Hit
CTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACCT221610.21703039816229772No Hit
CCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGG221270.2166974243101467No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT220220.21566912270791572No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT219710.21516966192968923No Hit
GGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCACGCCTACA219550.21501296835220643No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCCCACATGGACG218010.2135047926689343No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG214530.21009670735868297No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG208620.20430883834041133No Hit
GTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTC208320.20401503788263103No Hit
AGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCA206400.2021347149528372No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA199320.19520102414922244No Hit
CCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACC198790.1946819766738106No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT197040.19296814067009224No Hit
GGACAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTAGACGA194560.19053939021910854No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC192420.18844361362027584No Hit
CGGAGAGGCTCTGGAGAGGACCTTCGCGTCCTTCCCCACCACCAAGACCT190860.18691585123981835No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA186900.18303768519711855No Hit
GCAGCCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCAC182080.17831729117544864No Hit
CTCAGGTGCAGGCTGCCTATCAGAAGGTGGTGACCGGTGTGGCCAATGCC181780.17802349071766832No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA179910.17619213453083787No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG174200.17060013248441977No Hit
TGGCAGGCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGAC172210.1686512561144772No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG167250.1637937552125098No Hit
CGGCGCTGAAGAACTTGTCCAGGGAGGCGTGGACGGCGGGGGTGAATTCC163770.16038566990225847No Hit
CTGGTGTGCGTGCTGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGT163620.1602387696733683No Hit
CGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGC160230.15691882450045108No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC157250.15400040661983355No Hit
GTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCT154940.15173814309492537No Hit
CCCACATGGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCAC154350.15116033552795746No Hit
CCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCAC153660.15048459447506282No Hit
GTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGAGCACC153170.15000472039402168No Hit
GGCCAATGCCCTGGCTCACAAGTACCACTGAGACCCTGGCCTATTTCCTG153140.14997534034824364No Hit
CTTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCA152800.14964236649609267No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG151810.1486728249854177No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA150340.14723320274229432No Hit
GCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGG149510.14642035480910218No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCTTATATCGCGTAT148990.14591110068228302TruSeq Adapter, Index 9 (97% over 37bp)
CTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAG148660.1455879201787247No Hit
CAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGG145420.1424148752346976No Hit
CGGGAGGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCG144720.14172934083321026No Hit
GGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAG143300.14033868533305024No Hit
GCAACCCCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGC143060.140103644966826No Hit
TTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGC142330.13938873051956066No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT140630.1377238612588057No Hit
GTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAG140400.13749861424117413No Hit
TCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATG139110.13623527227271892No Hit
GGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTA137910.13506007044159776No Hit
CGGCGGGGGTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGG136380.1335616881069183No Hit
TTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCCA136000.1331895408603966No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC135400.13260193994483604No Hit
GGGAAACAGGGTCTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGT135180.13238648627579716No Hit
CAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCC134050.13127983788482472No Hit
CAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCAC132290.12955620853251373No Hit
GTCGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCA131200.12848873353591203No Hit
GCTGAAGGTTCTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTA130570.1278717525745734No Hit
TCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCC130340.12764650555694185No Hit
GGACGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCT128420.12576618262714803No Hit
CTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGGCGTT128180.1255311422609238No Hit
CTCAGGTGCAGGCTGCCTATCAGAAGGTGGTGGCCGGTGTGGCCAATGCC127740.12510023492284603No Hit
TGAAGGTTCTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATT126180.12357247254238855No Hit
CGGGCGAGTATGGCGGAGAGGCTCTGGAGAGAACCTTCGCGTCCTTCCCC125970.12336681222194235No Hit
CTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGA125720.12312197850712545No Hit
CGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGAC124670.12209367690489445No Hit
CCGCCGTCCACGCCTCCCTGGACAAGTTCTTCAGCGCCGTGAGCACCGTG123310.12076178149629047No Hit
CACACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGA122880.12034066750680539No Hit
CCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCA122790.1202525273694713No Hit
GGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGG122650.12011542048917384No Hit
GGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAAGGTTCTCTCCAGAG122580.1200468670490251No Hit
ACACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGA122020.11949843952783525No Hit
TGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTC121670.11915567232709157No Hit
GTCCATGTGGGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT121480.11896959870383074No Hit
CCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCA120530.1180392305875265No Hit
GTTCTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCC120450.11796088379878507No Hit
CGCTGACCAACGCCGTGGCCCACATGGACGACCTGCCGGGCGCGCTGTCC120440.1179510904501924No Hit
GTTATGAGCAACCCCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTC118520.11607076752039856No Hit
GGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCT118040.11560068678795012No Hit
GCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGCTTGA117660.1152285395414284No Hit
CCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACT117340.11491515238646276No Hit
CGGCAGGTCGTCCATGTGGGCCACGGCGTTGGTCAGCGCGTCGGCCACCT117250.1148270122491287No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG117200.1147780455061653No Hit
GGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGAGCAC116910.1144940383969777No Hit
TGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGTGCAGGCTGCCTAT116560.11415127119623403No Hit
GTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAA116060.1136616037666002No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC115860.11346573679474668No Hit
TGCTGGTGTGCGTGCTGGCTCACCACTTCGGCAAGGAATTCACCCCTCAG114520.1121534280833281No Hit
GTCAGCACGGTGCTCACGGCGCTGAAGAACTTGTCCAGGGAGGCGTGGAC114440.11207508129458667No Hit
GCGGAATTCACCCCCGCCGTCCACGCCTCCCTGGACAAGTTCTTCAGCGC113750.11139934024169201No Hit
GCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGG113560.11121326661843116No Hit
GTGGACTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGT113140.11080194597753874No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA113020.11068442579442665No Hit
TTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTT112850.11051793886835115No Hit
GCGACCTGCACGCCTACAAGCTGCGTGTGGACCCCGTCAACTTCAAGCTC112270.10994992464997592No Hit
GGCAGGCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACG111880.10956798405486155No Hit
CCTTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTC111340.10903914323085705No Hit
AGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGC111180.10888244965337422No Hit
GCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGCT111030.10873554942448407No Hit
CGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGA110640.1083536088293697No Hit
CGACCAGCAGCCTGCCCAGGGTCTCACCACCAACTTCGTCCACGTTCACC110060.10778559461099449No Hit
AAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACAC109790.10752117419899221No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC109650.10738406731869475No Hit
TGGGCCTTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTA109380.1071196469066925No Hit
GGACTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCT109080.10682584644891222No Hit
CGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCACTTCGACCTGAGCC108890.10663977282565136No Hit
ATTTGGAGGTCAGCACGGTGCTCACGGCGCTGAAGAACTTGTCCAGGGAG108720.10647328589957587No Hit
GTCGCTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGG108420.10617948544179559No Hit
CCGGTCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT108150.10591506502979331No Hit
CTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCT107650.10542539760015951No Hit
CCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGG107560.10533725746282543No Hit
GGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCA107340.10512180379378655No Hit
CTTCGGCAAGGAATTCACCCCTCAGGTGCAGGCTGCCTATCAGAAGGTGG107090.10487697007896964No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC107050.10483779668459894No Hit
CCCACTCAGACTTTATTCAGAGATCGGGAGGTTGAGGTGCGCGAAGCGCA106830.10462234301556006No Hit
GACGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTG106530.10432854255777978No Hit
GAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACA104600.10243842627939326No Hit
CCGGTGTGGCCAATGCCCTGGCTCACAAGTACCACTGAGACCCTGGCCTA104590.1024286329308006No Hit
AGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCAC103970.10182144531805466No Hit
GCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTT103960.10181165196946199No Hit
GCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGG103910.1017626852264986No Hit
GTCAAGTCCACCTGGGGCAAGATCGGCGGCCACGCGGGCGAGTATGGCGG103390.10125343109967944No Hit
GACACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTG103210.10107715082501127No Hit
CAGCACGGTGCTCACGGCGCTGAAGAACTTGTCCAGGGAGGCGTGGACGG102250.10013698936011436No Hit
GTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCAT102150.1000390558741876No Hit
GCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACC102120.10000967582840957No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)