FastQCFastQC Report
Fri 28 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P3_C11_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P3_C11_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8196073
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC145340.17732882564613564No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGCTCGATCTCGTAT117540.1434101428818411TruSeq Adapter, Index 6 (97% over 36bp)
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG107910.13166061356456926No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC99330.12119218557472586No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)