FastQCFastQC Report
Thu 20 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P3_B3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P3_B3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACCAGCGGTTCGAGAAAGTGTCCTACTTTGTGTTTGGCGATTTCAACT14.0No Hit
CAACGCGCACCAGTAAAAACTACACCAACAGCAAAAAAACAACGCCTCCA14.0No Hit
AAGAACTCATAATCATCCTACTATAGCAATAACATCAATAATATCACCTG14.0No Hit
AACTAACAAACATATAACAACCACCACAACACAAGCTCCACCATAAACCA14.0No Hit
CACAAATCATCACAGCATGGGGAAAAGCGGGCACACGGGAAAGACCCAAA14.0No Hit
TGCCCACAAAGTTAAGATTATACAAACCAACAATTCATATATCATAATCT14.0No Hit
CCACGGCAGAGACATTGAGGATACCATTAGAATAAATATCGAAAGTTACC14.0No Hit
CTGTGACCCTTTTTGAGGTCAGACTGGGAGACAGTATGGTCAGTTTCTCG14.0No Hit
GTGGGAATCAGTGGACGAATCCACCTATAATTGCAAATACTGCCCCTATT14.0No Hit
TCCACGTACAATGACAATAAATTAAATACCACAACCACAACACGAATGAC14.0No Hit
TGGGCATGAGGTCCTCGGGGCTGGCTGATGCCCGGGCCCCTGGAAGTGAT14.0No Hit
GGTCCCGCCGCTCTCACCCCCACTATGCAAGCTGCCACCGGGACAAGGAC14.0No Hit
CTTGAGACTTGAAGGGAAGCAGGGGAGGCCGAAACGGCCCTGGGGGGCTG14.0No Hit
CACGATATTCCAGGTAATGGGTGAGAGACACAAAGTTCTAAAGGTCCACC14.0No Hit
GTTAATGTCACATTTCAGATGGAATCCACAATCACAAGGATTGATTATGT14.0No Hit
TGTTGAGGTACTTGTAGGTATAGCCAGGGTTGCCGATGAGGATGCGCGAC14.0No Hit
GCTCTACCTACCTGTCCTTGACCTTGGTTCTTCAAACATTACCTGGCAGG14.0No Hit
TCTACATTGAACGAGTGCAGCGAGAGAAGGCGAATGGCACAACCGTCCAT14.0No Hit
CCGCAGTAATCAGTATCTCCTATACGCCGATGCTGAGCTGCCGTCTGAGA14.0No Hit
AACAAAACCACCATCAACAAACATGAGACAACCAACCGCATGATCATTTA14.0No Hit
CTATTCACATGAAAAATAAAGCAATCACTTAAACAACAAGTCAAGCATAA14.0No Hit
TCTGACTACGTGAAGAATTATATCTACCAGGGAAAAAGGATACACCCTGA14.0No Hit
TGAAAAAACACAACATAAAAAAAACAGAAAAAAATAAAAAAACATTTTAA14.0No Hit
ACTAGCCCTACCTACTCGCCCACCACCCCAAAGTACTCCCCCACATCACC14.0No Hit
GCGGCGTCCAACATGGAGCTACACGCCCTGTCAACGGGCCGCGAGCCTCG14.0No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)