FastQCFastQC Report
Thu 20 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P3_A8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P3_A8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAAGTTGTAGTAAATAAAATCAGTGCACAGAAGTCAGTTGCATTTCTA40.27548209366391185No Hit
TTCAAACCCACGTTATTGGCTAAAACGCCGGCCACTGCTAGAGCGGTCAG30.2066115702479339No Hit
TGGACATCAGCATTCTTCTTAAGAATGCTTTTGAGACAAAACACTTAACT20.13774104683195593No Hit
CAACAGTGTGAGGTGGCTGTAAGATGTGCTCACTCCCAACATAGCCAGAG20.13774104683195593No Hit
TCATGATACCCTCCTCTGTATTCTGTGTTGTTTCTCCAGTCACTCAGGTC20.13774104683195593No Hit
TTAACCCAGCCCCAGGATCCCTGGGGTTTGGAGAGAGGTTTTTCTCACCT20.13774104683195593No Hit
GAGGGGAGGGTGTCCACCAGATTCCTCAGCTGGATCATCCCATCTCTCAT20.13774104683195593No Hit
CTGCACTCCAGGCCGGATTAGATTTTTGTCCATCTCCCCAGTTCTGAGAT20.13774104683195593No Hit
ATGGACTCCAACTGCCCACAAAGCAGGTTCAAGTCCTGCACCGGACGACT20.13774104683195593No Hit
CTTCTCTCTTGATCTTCATCTGCAGTCCTTTGCCCCTTATTTGGATGCCT20.13774104683195593No Hit
GCTGGCTGCTGCCAGAACCGCTGCTGTCTCTGCTGCCGGCTCTGCTGCCG20.13774104683195593No Hit
CGCTGTGCCCCCTGGTGCCGACAAGAAAGCTGAGGCTGGGGCTGGTTCAG20.13774104683195593No Hit
TCTGGCTGTAGTTGGCGCCGGCGGCGGCCAGGGCCCCGACGGGGGCGGCG20.13774104683195593No Hit
TTCATCCAGTCCCTGCACCTGCTTCTACCATAGAGGAAGCTAGTAGGAGA20.13774104683195593No Hit
TGTGACTAGACTTTTAAACTTTGCTCTTTCAAGCAGAAGCCTGGTTTCTG20.13774104683195593No Hit
CTTCTTATCCCTTTCTAAAAGCCCTCCAGTCTCTTCCTGATTTTTGGTGT20.13774104683195593No Hit
GTAAAAGCCTGCAAACGCTAACATTGCTTAAGAGAATCTGTAAACGCGTT20.13774104683195593No Hit
CCAAACCCATAGCTGGTAATTCAATGGCTCAGGAAAGGGGGGAGCCCGGC20.13774104683195593No Hit
CGGAGCTGCAGCAAGCTCTGTCCCAGATGGGCTACAACCTGAGCCCCCAG20.13774104683195593No Hit
AGAAGGAGGAAGGAAGGAGTGACAATAATTTTATAATCTATTTTATGTAT20.13774104683195593No Hit
AGAGCGTGCCTCCTGTAGTACGTAGATGAGGAAGCAGAGGCACAGAGATG20.13774104683195593No Hit
CCGGAAGTCGCCGAGAGGCGCGGAGATGGACAGGGCGCCGCGCTCAGGCC20.13774104683195593No Hit
TTTTAATATTTTTGTTTTAATATGAATAGTAACATAGTTTATGGGTTTGG20.13774104683195593No Hit
CGAGCGACCAAAGGAACCATAACTGATTTAATGAGCCATTCGCAGTTTCA20.13774104683195593No Hit
GAGATTTTCAGGCAGAAGGAGAGGGCCATGTCCACCACATCCATCACAAG20.13774104683195593No Hit
GGGGTATGGTGGCCTTGAAATGTTCCTTCTCGGACCACATCTCGTCACCA20.13774104683195593No Hit
TGCCGGGTCTCCCTCCACAGAGCGTGCCCCAGAGCGGCGGGACGTGTGCT20.13774104683195593No Hit
GTTCTTTGGCTTGATCTTCCTGAACTCGTCACAGTCACAGAGGATCAAAT20.13774104683195593No Hit
TTTTAAGAATTCACCAGAAGCCTGAATTTCTAAGTGTTGGACATTTGGAG20.13774104683195593No Hit
GAAGAAGTACGGCTGTAATTAAAACTGATCAAACAAATAGTGGAGTTTGG20.13774104683195593No Hit
CGAGAAGTCAGAATGCACGTGGATGGGTGGCCAGAACGCACCGAGGAGTC20.13774104683195593No Hit
ACTTATCACCCACCTTCGAAGTTTTAAAAATATTTATTCAATAGATATTT20.13774104683195593No Hit
CGGGATCCCCAGCATGGTCAGCATAAACTTCAGGGGATTGAAGATTCCGA20.13774104683195593No Hit
GTTGCATGGCTCGGCAGGTCCTGTTCAGCTCCTGTAGCAATTCCAGAGAT20.13774104683195593No Hit
CGGCCGTCGCGGACCTGCCTGCGAGAATGGCGGGTAGGGGGAAGCTAATC20.13774104683195593No Hit
GCGGCACTCGCGTGTCAGGCGGTTGCTAGGCTCCGGCCGCGCGCCCCGCC20.13774104683195593No Hit
GGAAGGATGGCATATCTTTCCAGCTGAAAAAATCCTGGCAAACTACAAGC20.13774104683195593No Hit
TTTCTGAATGAAGTAACTTCTCATATTCACTGAAGAATTTATCATAATGT20.13774104683195593No Hit
TACTAATGTTTCCTGTTTGCTATATTCTTTTTCTTAACCATTTGGCCATC20.13774104683195593No Hit
CTAACACGTGCGCTCATGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGG20.13774104683195593No Hit
GTCGGAGTCAGATGGAATTCCGGAGGGGTTATTAGATCCTGTTTCGTGGA20.13774104683195593No Hit
CGAAAATGTTGAGCAACTGGTTCCTGTAGGCCGAGCACATGAGGAGGGTG20.13774104683195593No Hit
GGCCATGAATCCTGTGGAGCATCCTTTTGGAGGTGGCAACCACCAGCACA20.13774104683195593No Hit
TATTAAGCCACTCAGCTTGCCTCTGTCTTATTGTTAATACTTTCCAGGTT20.13774104683195593No Hit
GTTCTGAGGTCATACCCAATGTGACCAGACTGATCATTCCCACCCTGATG20.13774104683195593No Hit
TAAGCCTTCTGCACAGCACAAGCATGATGGCCTGGGTGGCGCTTTTGGCC20.13774104683195593No Hit
AAAAGCCTCTTTAGCCCACTTTCTACCACAAGGAACCCCACTACCCCTAA20.13774104683195593No Hit
AAAGCTGTACTATTCTGAATATATGAATAGGTTGAAGGGAACAGAGATGA20.13774104683195593No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13774104683195593No Hit
GGGCACAGGGCTACCCCCCCAAGGCCATCCATCGTCCATGGAGGGCTTCT20.13774104683195593No Hit
ATCCTTTCCAGGTATTGAGAGGTTCAAAGGCCAGTATTTCCATAGCCACC20.13774104683195593No Hit
TTTGCAGGTTAAACGTACAGGCCTGACTTCTTAAAAAAACAAAAACAAAA20.13774104683195593No Hit
CTGCTGTCGGGGCAGAGAGGATCGGCTCCAGGTGGGCTGGCGTCTGATGG20.13774104683195593No Hit
ACTGGGGTCCTGTTGATCTCAAATGAGAATTCCACAAAACAAAGGCAGAT20.13774104683195593No Hit
GCCAGGACATCCAAGGCAAAGCGCACAGTGTCATTCCTTGCATAATAGGT20.13774104683195593No Hit
CAAACATCAAGACGGAAGAGATCTCTGAGGTGAAGATGGACGCCGAGTTC20.13774104683195593No Hit
AAGAAGTGTGGCAAGCACCAACCCCACAAAGTGACACAGTACAAGAAAGG20.13774104683195593No Hit
GTCAGATGAGACACACAGCCCGGGGCCCCTCTCGCTGGTGCTTGGCTGGA20.13774104683195593No Hit
CTTTTCTTTAGAAATATAATAGCTGCCTTAGACATTATGAGGTATATAAC20.13774104683195593No Hit
TTTATGTCTTATTTTTACACTGTCAGAAGCACTCAGTAAACCTAGCAACA20.13774104683195593No Hit
CGCTCTGCTGTCTCTCTTTTGGCCTGCTCCTCTGCTAAGGCTCTTTGTAT20.13774104683195593No Hit
CCTGAGTCCTTCGTAACCTTGGTCGGGACGTGGCCGTTAGACAGGGAGGG20.13774104683195593No Hit
GTCTTGGTTACAAGGAAAACAGATTCCACTAATGGAGCCCAAGTTGCAAA20.13774104683195593No Hit
GGCAGAGCCACTGACGGAGCTGGAAGCGGCCATTGAGACGGTGGTCACCA20.13774104683195593No Hit
GTTGTCTTAGCTCCAGAAATTCTCAAGAATTTAAATAAAGAAACTCGGGG20.13774104683195593No Hit
CCCTGCATGACCCATTCTCCTGCCAGGAGGAGCAGTTAAACCAGCGATGA20.13774104683195593No Hit
AAAAACTATGAAACAGATGCATATTTCTTCAACATATTGTGTCAGATACA20.13774104683195593No Hit
CTGACTGCAAGGTTTTCCCCAGGGCTCCCAAACCGCTGCTCAGCGGGGGA20.13774104683195593No Hit
ACAGATTCTGCCCAGGAAGAGTATTGCTTTTTGTTTCCAGCAGGGTTGTT20.13774104683195593No Hit
CTGAGGGTCACATTGAGCTGCGAGGTGCTGCTGTGGTGTCTTTAGAGTCA20.13774104683195593No Hit
CCCAGTGATGACTTCCACTTTTTCCAGCAAACACTTCCGTGCTGTTGCCT20.13774104683195593No Hit
CAGATACTCATCAGTTGGATTTTTTCCTAAGGAGGCAAGCATATCATGCA20.13774104683195593No Hit
CCTGGATGGAACCCCTGAGCCCTTTTGAGGATGTGGCTGGAACTGAGATG20.13774104683195593No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)