FastQCFastQC Report
Fri 28 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P2_H12_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P2_H12_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44435
Sequences flagged as poor quality0
Sequence length151
%GC75

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGTAT1421131.98154607854169TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGCGTAT714616.081917407449083TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGTTT30716.911218633959717TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGGAT23375.259367615618319TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGCGGAT17743.9923483740294814TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGCGTTT12042.709575784854281TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGGTT8581.930910318442669TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGTGT7051.5865871497693258TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGCGTGT5431.2220096770563744TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGCGGTT4941.1117362439518397TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTGGTAT3890.8754360301564081TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTGGTTT2350.5288623832564421TruSeq Adapter, Index 10 (97% over 38bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGTA1990.44784516709800837TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGCGGGT1930.4343422977382693TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGT1740.3915832114324294TruSeq Adapter, Index 10 (97% over 38bp)
CTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGT1670.3758298638460673TruSeq Adapter, Index 10 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGTATG1490.33532125576685046TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGGGTAT1490.33532125576685046TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGGGT1450.3263193428603578TruSeq Adapter, Index 10 (97% over 38bp)
ATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1350.30381456059412626Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGTA1340.3015640823675031TruSeq Adapter, Index 10 (97% over 38bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGCGTA1060.23855069202205467TruSeq Adapter, Index 10 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGCGTATG990.22279734443569257TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTGGGAT920.2070439968493305TruSeq Adapter, Index 10 (97% over 38bp)
GCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACC890.20029256216946104TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGCGTCT750.1687858669967368TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACC730.1642849105434905TruSeq Adapter, Index 10 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGCGTA710.15978395409024418TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTGGTGT670.15078204118375155TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGGGTTT630.14178012827725892TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGTAA620.13952965005063575TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGTCT590.13277821537076628TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTGGGTT570.12827725891751995TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCGGGGAT560.12602678069089682TruSeq Adapter, Index 10 (97% over 38bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGTGATCTCGTT520.11702486778440419TruSeq Adapter, Index 10 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)