FastQCFastQC Report
Fri 28 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P2_E2_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P2_E2_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397428
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGATCATCTCGTAT268166.747385689986614TruSeq Adapter, Index 18 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGATCATCGCGTAT98632.481707378443391TruSeq Adapter, Index 18 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGATCATCTCGTTT17160.43177632174884506TruSeq Adapter, Index 18 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGATCATCTCGGAT8320.20934609539337945TruSeq Adapter, Index 18 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGATCATCGCGTTT5540.13939631832684157TruSeq Adapter, Index 18 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGATCATCGCGGAT5530.13914470042372454TruSeq Adapter, Index 18 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)