FastQCFastQC Report
Fri 28 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P2_E12_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P2_E12_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21548571
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC984120.45669849754770275No Hit
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG745370.34590228744170554No Hit
GGGAGGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGA692320.32128348557312686No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC653780.30339830887161845No Hit
CGGAGAGGCTCTGGAGAGAACCTTCGCGTCCTTCCCCACCACCAAGACCT606070.2812576295662483No Hit
GGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCG604880.2807053887703273No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA601040.2789233680507167No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC600490.2786681307080641No Hit
GGGTCTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTG599980.2784314560812408No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG581830.2700086237737064No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC573500.2661429382022594No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC554180.25717714645671863No Hit
GGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCACGCCTACA536330.24889353451790375No Hit
GAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCA502830.23334726001088424No Hit
GGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACA495510.22995028301412654No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCCCACATGGACG495140.22977857789270575No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA452220.2098607838078915No Hit
CGGAGAGGCTCTGGAGAGGACCTTCGCGTCCTTCCCCACCACCAAGACCT444330.20619928811056656No Hit
GAAGAATCTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGC430480.1997719477546794No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC420590.19518231626589067No Hit
GGACAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTAGACGA416790.19341885826210936No Hit
GTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCC410520.1905091525558702No Hit
CTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACCT399570.18542760909760558No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA399320.1853115921236726No Hit
CTGGTGTGCGTGCTGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGT398600.18497746323874562No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA392430.18211416432207964No Hit
CCCACATGGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCAC371410.17235945715379455No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG368710.17110647383531838No Hit
GTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTC368120.17083267377683653No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA347950.1614724243199236No Hit
GTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAG345570.1603679427280816No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG343910.1595975900211666No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA343350.15933771199955674No Hit
GGGAAACAGGGTCTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGT341290.15838173213434895No Hit
TGGCAGGCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGAC341100.1582935592341599No Hit
CGGCGCTGAAGAACTTGTCCAGGGAGGCGTGGACGGCGGGGGTGAATTCC340960.15822858972875742No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG333420.1547295177949387No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC332280.15420048039380432No Hit
CTTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCA332130.15413087020944452No Hit
CGGCGGGGGTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGG329360.15284540213826708No Hit
CCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGG326880.15169451375685192No Hit
AGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCA325060.1508499101866198No Hit
GTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCT319230.14814439435450266No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA316810.14702135004683142No Hit
CGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGC316740.1469888652941302No Hit
GGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAG314160.1457915701231418No Hit
GCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGG312810.1451650784639037No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA307920.1428957864537746No Hit
TCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCC307720.1428029728746282No Hit
CAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCAC306560.14226465411557918No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT306540.14225537275766453No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA300240.1393317450145534No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT298740.1386356431709555No Hit
GCAGCCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCAC298430.13849178212327862No Hit
CCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACC297620.13811588712773576No Hit
CGGGAGGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCG283130.13139154331858016No Hit
GGCCAATGCCCTGGCTCACAAGTACCACTGAGACCCTGGCCTATTTCCTG282060.130894990670147No Hit
CTCAGGTGCAGGCTGCCTATCAGAAGGTGGTGACCGGTGTGGCCAATGCC281720.13073720758559815No Hit
CGGGCGAGTATGGCGGAGAGGCTCTGGAGAGAACCTTCGCGTCCTTCCCC281700.1307279262276835No Hit
TGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGTGCAGGCTGCCTAT278820.12939141068797555No Hit
CGACGCGCTGACCAACGCCGTGGCCCACATGGACGACCTGCCGGGCGCGC275990.12807809854305421No Hit
GGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAAGGTTCTCTCCAGAG272910.12664876942419986No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT271240.12587377603832756No Hit
GCGGAATTCACCCCCGCCGTCCACGCCTCCCTGGACAAGTTCTTCAGCGC269010.12483890463084535No Hit
GGCAGGCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACG264670.12282484996336882No Hit
GGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGAGCAC263420.12224476509370388No Hit
GTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGAGCACC262120.12164147682925239No Hit
CTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGGCGTT261670.12143264627617302No Hit
CGCTGACCAACGCCGTGGCCCACATGGACGACCTGCCGGGCGCGCTGTCC260250.1207736698642337No Hit
CTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGA259840.1205834020269836No Hit
GGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTA258800.12010077141542241No Hit
CAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCC258540.11998011376253209No Hit
CCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCA255830.11872248976509858No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC253000.11740917762017723No Hit
GGCCGACGCGCTGACCAACGCCGTGGCCCACATGGACGACCTGCCGGGCG252880.11735348947268939No Hit
GCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGCTTGA250130.1160773027594266No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG248070.11512132289421882No Hit
CTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATG244960.11367807173849254No Hit
GGACGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCT244100.11327897334816309No Hit
CCTTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTC242700.1126292782941384No Hit
GCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTT240730.11171506453954651No Hit
GCGGGGGTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCA239420.11110713559613769No Hit
CTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAG238560.11070803720580821No Hit
CGACCAGCAGCCTGCCCAGGGTCTCACCACCAACTTCGTCCACGTTCACC238180.11053169140543008No Hit
GCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGG237260.11010474894135672No Hit
CCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTAT235940.10949217931899057No Hit
CCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCC234190.1086800605014597No Hit
GCAACCCCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGC233080.10816494513719727No Hit
GGACTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCT230890.10714863644554434No Hit
TTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCCA229370.10644325324403181No Hit
CCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCA228440.10601167010100114No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC228410.10599774806412916No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCACCACC228210.10590493448498278No Hit
GTCAAGTCCACCTGGGGCAAGATCGGCGGCCACGCGGGCGAGTATGGCGG228120.1058631683743669No Hit
GGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGG227840.10573322936356197No Hit
CAGCACGGTGCTCACGGCGCTGAAGAACTTGTCCAGGGAGGCGTGGACGG227550.10559864967379971No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC225930.10484685968271398No Hit
GTCAGCACGGTGCTCACGGCGCTGAAGAACTTGTCCAGGGAGGCGTGGAC224280.10408114765475632No Hit
CTGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGTGCAGGCTGCCTA223160.10356139161153656No Hit
GGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAAGGTTCTCTCCAGA222020.10303235421040217No Hit
CTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCT220640.10239194051429211No Hit
CCGGTGTGGCCAATGCCCTGGCTCACAAGTACCACTGAGACCCTGGCCTA220240.10220631335599933No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC219040.10164943188112102No Hit
CCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCAC215730.10011336714624834No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)