FastQCFastQC Report
Fri 28 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P2_D8_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P2_D8_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160682
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGCATAATCTCGTAT116050.9998431956384264TruSeq Adapter, Index 12 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGCATAATCGCGTAT44690.38503224828161375TruSeq Adapter, Index 12 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGCATAATCTCGTTT44610.38434299834063074TruSeq Adapter, Index 12 (97% over 39bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26410.22753863676700423No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGCATAATCTCGGAT16890.14551789379003036TruSeq Adapter, Index 12 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGCATAATCGCGTTT13600.11717248996710554TruSeq Adapter, Index 12 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)