FastQCFastQC Report
Thu 20 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P2_D6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P2_D6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196823
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCTCGTAT2527412.840978950630769TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCGCGTAT153367.7917723030336905TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCTCGTTT3080.15648577656066617TruSeq Adapter, Index 25 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCTCGTA2540.1290499585922377TruSeq Adapter, Index 3 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCTCGTATG2490.12650960507664247TruSeq Adapter, Index 25 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCTCGTA2220.11279169609242823TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGAACATCTGGTAT2110.10720291835811871TruSeq Adapter, Index 25 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)