FastQCFastQC Report
Fri 28 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P1_H7_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P1_H7_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186007
Sequences flagged as poor quality0
Sequence length151
%GC79

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGGTT3801920.439553350142738TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGGGT185039.947475095023306TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGTTT165818.914180649115355TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCGCGGTT158608.526560828355922TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCGCGGGT134627.237362034762132TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGGAT85004.569720494389997TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGTGT67693.6391103560618685TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGTAT61593.3111657088174105TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCGCGTTT51302.7579607219083155TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCGCGGAT41272.2187337035702956TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCGCGTGT37181.9988495056637654TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCGCGTAT23451.2607052422758285TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGGT16920.9096431854715145TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGGTG10530.566107727128549TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGGG10530.566107727128549TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTGGGTT9980.5365389474589666TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCGCGGT9960.5354637191073455TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCGCGGG7770.41772621460482673TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAG6790.3650400253753891TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGTT6300.33869693076067026TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTGGTTT6260.33654647405742794TruSeq Adapter, Index 22 (97% over 37bp)
CTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAG6260.33654647405742794TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGGGG6210.333858403178375TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTGGGGT5770.31020337944270915TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCGCGGGG5400.2903116549377174TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCGCGGTG5130.2757960721908315TruSeq Adapter, Index 22 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGGT4250.2284860247194998TruSeq Adapter, Index 11 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGTG3560.19139064658856925TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCGGGGGT3280.17633744966587278TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCGGGGTT3100.1666603945012822TruSeq Adapter, Index 22 (97% over 37bp)
ATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3070.16504755197385043Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGGA2640.14193014241399515TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTGGTGT2630.1413925282381846TruSeq Adapter, Index 22 (97% over 37bp)
GCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTA2620.140854914062374TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGGCT2610.1403172998865634TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGTTG2520.1354787723042681TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTA2450.13171547307359402TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGGAG2330.12526410296386695TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCGCGGCT2280.12257603208481402TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGTA2210.11881273285413989TruSeq Adapter, Index 22 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGGTTG2180.11719989032670813TruSeq Adapter, Index 22 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCGCGTT2160.11612466197508696TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCTAGGATCGCGGGT2120.11397420527184461TruSeq Adapter, Index 9 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCGCGGT2020.10859806351373874TruSeq Adapter, Index 11 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGGAA1900.10214669340401167TruSeq Adapter, Index 22 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)