FastQCFastQC Report
Fri 28 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P1_E6_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P1_E6_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7257985
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCTCGGTT531890.7328342508285701TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCTCGGGT326010.44917425428683033TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCTCGTTT169160.2330674422722009TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCGCGGGT95150.13109699179593234TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCTCGTGT91580.12617827124194939TruSeq Adapter, Index 10 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89630.12349157514103433No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCGCGGTT86260.11884841316150418TruSeq Adapter, Index 10 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)