FastQCFastQC Report
Thu 20 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P1_D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P1_D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251084
Sequences flagged as poor quality0
Sequence length151
%GC80

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGGGT7536530.015851268898057TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGGGG4953219.72726258941231TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCGCGGGT211238.412722435519587TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCGCGGGG164516.551990568893279TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGGTT156396.228592821525864TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGGTG85823.417979640279747TruSeq Adapter, Index 19 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGGG33821.3469595832470407TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCGCGGTT25651.0215704704401716TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCGCGGTG19900.7925634449029011TruSeq Adapter, Index 19 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGGG12380.4930620828089404TruSeq Adapter, Index 18 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCGCGGG11450.45602268563508624TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGTGT11110.4424814006467955TruSeq Adapter, Index 19 (97% over 37bp)
CTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCG7270.28954453489668797TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCG7130.2839687116662153TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTGGGGT6980.2779946153478517TruSeq Adapter, Index 19 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGGT5260.20949164423061606TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGTGG5220.20789855187905243TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGACTCTCGGGG4960.19754345159388892TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGTTT4480.17842634337512547TruSeq Adapter, Index 19 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCGCGGG4160.1656816045626165TruSeq Adapter, Index 18 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTGGGGG4040.16090232750792563TruSeq Adapter, Index 19 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGGGTG3530.14059040002548948TruSeq Adapter, Index 19 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGACTCGCGGGG3350.1334214844434532TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGACTCTCGGGT3200.12744738812508963TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGGAT3090.12306638415828966TruSeq Adapter, Index 19 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGGGGG2970.11828710710359878TruSeq Adapter, Index 19 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCGCGTGT2860.11390610313679883TruSeq Adapter, Index 19 (97% over 37bp)
ATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2830.11271128387312614Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAA2750.10952509916999889TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGACTCGCGGGT2550.10155963741218078TruSeq Adapter, Index 19 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)