FastQCFastQC Report
Fri 28 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P1_C7_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P1_C7_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162610
Sequences flagged as poor quality0
Sequence length151
%GC76

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCTTATCTCGTAT7571146.55986716684091TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCTTATCGCGTAT4430827.248016727138552TruSeq Adapter, Index 16 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCTTATCTCGTA35212.1653034868704264TruSeq Adapter, Index 16 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCTTATCGCGTA23901.4697743066232087TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCTTATCTCGTTT9590.5897546276366767TruSeq Adapter, Index 16 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCTTATCTCGTA9030.555316401205338TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCTTATCTCGGAT8530.5245679847487855TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCTTATCGCGGAT8410.5171883647992128TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCTTATCTGGTAT7440.45753643687350104TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCT6250.38435520570690607TruSeq Adapter, Index 16 (97% over 37bp)
CTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCT6070.37328577578254724TruSeq Adapter, Index 16 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCTTATCGCGTA5390.33146792940163583TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCTTATCGCGTTT4480.2755058114507103TruSeq Adapter, Index 16 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCTTATCTCGTATG3990.24537236332328885TruSeq Adapter, Index 16 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCTTATCGCGTATG3620.22261853514544003TruSeq Adapter, Index 16 (97% over 36bp)
ATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3100.1906401820306254Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCTTATCGGGTAT3090.19002521370149436TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCGTATCTCGTAT2480.15251214562450033TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACCAGTGCTTATCTCGTATG2380.14636246233318984Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGT2380.14636246233318984TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCTTTATCTCGTA2220.13652296906709305TruSeq Adapter, Index 16 (97% over 37bp)
GCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGT2030.1248385708136031TruSeq Adapter, Index 16 (97% over 37bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCTTATCTCGTAT1840.11315417256011315TruSeq Adapter, Index 7 (97% over 36bp)
CCTTAATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGG1830.1125392042309821No Hit
GCCTTAATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGG1780.10946436258532685No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTGCTTATCTCGTGT1670.10269971096488531TruSeq Adapter, Index 16 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)