FastQCFastQC Report
Thu 20 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P1_C1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P1_C1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7727164
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCAGGTATCTCGTAT527320.6824237197502214TruSeq Adapter, Index 18 (97% over 37bp)
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG516650.668615290163377No Hit
GGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACA396410.5130083948004728No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCAGGTATCGCGTAT373220.48299738429260725TruSeq Adapter, Index 18 (97% over 37bp)
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA334820.43330256741024264No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC313780.40607394899344706No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC301370.3900137230166203No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC284190.3677804690051874No Hit
GGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCG269850.3492225608256794No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC261100.3378988720829531No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT249760.3232233714723798No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA240280.310954963554546No Hit
GGGTCTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTG235460.30471722872712426No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC231180.2991783272621107No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA229730.29730183027045887No Hit
CGGAGAGGCTCTGGAGAGAACCTTCGCGTCCTTCCCCACCACCAAGACCT226380.29296647515181506No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCCCACATGGACG224840.29097350593309523No Hit
GGACAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTAGACGA222390.28780287308513186No Hit
GGGAGGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGA216800.28056865364835015No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC212660.2752109312032202No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC209180.27070733842325595No Hit
GAAGAATCTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGC200870.25995306945730673No Hit
GGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCACGCCTACA194120.25121765242720356No Hit
GTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCC189010.2446046182014514No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA188300.24368578174347016No Hit
CTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACCT185780.240424559385565No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA185520.240088084062924No Hit
CGGAGAGGCTCTGGAGAGGACCTTCGCGTCCTTCCCCACCACCAAGACCT182620.23633509007962042No Hit
CTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGA179890.23280209919188982No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA178980.23162443556264625No Hit
GTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTC174870.22630553719320567No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG172930.22379491363196122No Hit
GAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCA166590.21559009230294582No Hit
GCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGG165260.21386889161405143No Hit
TGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGTGCAGGCTGCCTAT162020.2096758914396019No Hit
TCTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCG156830.2029593263453448No Hit
GGGAAACAGGGTCTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGT156770.2028816781939661No Hit
TGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGAC153840.19908986013497318No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT151650.19625570260965083No Hit
GTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAG151010.1954274556616114No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG151000.19541451430304832No Hit
GTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAG149390.19333095557438668No Hit
GCAGCCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCAC146090.18906030724855846No Hit
AGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCA145380.18814147079057725No Hit
GGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAG144660.1872096929740329No Hit
TTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCCA143260.1853979027751967No Hit
TCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCC136330.17642954129095745No Hit
GTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCT136310.17640365857383122No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA134510.1740742140324704No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG133900.17328479116012033No Hit
CCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCA131510.17019180646353566No Hit
CTGGTGTGCGTGCTGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGT131440.17010121695359384No Hit
CGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGA130850.16933767679837003No Hit
GGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCT130050.16830236811332075No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT129820.16800471686636909No Hit
GGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTA129050.16700823225700917No Hit
TGGCAGGCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGAC127090.1644717259786385No Hit
CCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGG126480.16368230310628842No Hit
GACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAA126430.16361759631347284No Hit
GGCCAATGCCCTGGCTCACAAGTACCACTGAGACCCTGGCCTATTTCCTG126280.1634234759350261No Hit
CGGCGGGGGTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGG125090.1618834542660153No Hit
CTTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCA124980.16174109932182104No Hit
CAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCAC124470.16108109003510215No Hit
CTCAGGTGCAGGCTGCCTATCAGAAGGTGGTGACCGGTGTGGCCAATGCC121910.15776810224294452No Hit
GCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGCTTGA119140.15418334592096145No Hit
GCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTT118540.1534068644071745No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG118340.15314803723591217No Hit
TTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTCTGATAGGCAGC115460.14942092596973483No Hit
CGACCAGCAGCCTGCCCAGGGTCTCACCACCAACTTCGTCCACGTTCACC114020.14755737033664615No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG111140.1438302590704688No Hit
CCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACC109630.14187611392743832No Hit
GGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCACCGC109460.14165611083186536No Hit
GCTGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGTGCAGGCTGCCT109360.14152669724623418No Hit
CTGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGTGCAGGCTGCCTA108000.13976667248165045No Hit
CGGCGCTGAAGAACTTGTCCAGGGAGGCGTGGACGGCGGGGGTGAATTCC107670.13933960764906764No Hit
GCGGGGGTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCA105800.136919573597765No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC105470.13649250876518215No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC105380.13637603653811411No Hit
GGCAGGCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACG104030.13462895313209347No Hit
CTGGAGAGGACCTTCGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCA103760.13427953645088936No Hit
CCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACT102890.1331536382558983No Hit
TGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTG102060.13207950549515968No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT101940.13192420919240228No Hit
CCCACATGGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCAC101890.13185950239958671No Hit
GGAGAGGCTCTGGAGAGAACCTTCGCGTCCTTCCCCACCACCAAGACCTA101850.13180773696533424No Hit
CTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCT100870.13053948382614888No Hit
AAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACAC100350.12986653318086688No Hit
TTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGC99980.1293877029140316No Hit
CAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGG99690.12901240351570123No Hit
ATCTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGC97920.12672178305002973No Hit
CCGCCGTCCACGCCTCCCTGGACAAGTTCTTCAGCGCCGTGAGCACCGTG97850.12663119354008795No Hit
GCGGAATTCACCCCCGCCGTCCACGCCTCCCTGGACAAGTTCTTCAGCGC97020.1255570607793493No Hit
GCAACCCCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGC95400.12346056069212456No Hit
AGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGC94970.12290408227391059No Hit
CTTCGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCACTTCGACCTGA94550.12236054521425972No Hit
CCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCA94320.12206289396730806No Hit
GATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCC94290.12202406989161871No Hit
CGACGCGCTGACCAACGCCGTGGCCCACATGGACGACCTGCCGGGCGCGC93850.1214546501148416No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA93850.1214546501148416No Hit
CGCTGACCAACGCCGTGGCCCACATGGACGACCTGCCGGGCGCGCTGTCC92650.11990168708726771No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG91020.11779224564147985No Hit
GAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACA90980.11774048020722738No Hit
CCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCCC90680.1173522394503339No Hit
CGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGC90470.11708047092050848No Hit
GCGACCTGCACGCCTACAAGCTGCGTGTGGACCCCGTCAACTTCAAGCTC89950.11640752027522645No Hit
CTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGA89670.11604516223545921No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC89450.11576045234707066No Hit
TTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAG89360.11564398012000263No Hit
CTCAGGTGCAGGCTGCCTATCAGAAGGTGGTGGCCGGTGTGGCCAATGCC89210.1154498597415559No Hit
CTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATG89050.11524279800454605No Hit
CTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAG88420.11442749241506973No Hit
GCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTT88300.11427219611231236No Hit
GGACGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCT88180.11411689980955497No Hit
GGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCGGCAAGGAATTCA87650.11343100780570982No Hit
GGGTAGACGACCAGCAGCCTGCCCAGGGTCTCACCACCAACTTCGTCCAC86270.11164510032399987No Hit
TTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAG86130.11146392130411624No Hit
GAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTT86110.11143803858698999No Hit
ATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCCAGTG86010.11130862500135885No Hit
GCGTCGGCCACCTTCTTGCCGTGGGCCTTGACCTGGGCGGAGCCGTGGCT85750.11097214967871784No Hit
GCAGTTTGTCGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGG85130.11016978544780465No Hit
CCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGG84930.10991095827654233No Hit
GTCGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCA84640.10953565887821198No Hit
GGAGAGGCTCTGGAGAGGACCTTCGCGTCCTTCCCCACCACCAAGACCTA83580.10816387487052172No Hit
TTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTT83430.10796975449207497No Hit
CTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGGCGTT82950.10734856928104541No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA82900.10728386248822983No Hit
GGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAAGGTTCTCTCCAGAG82880.1072579797711036No Hit
CTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGT82870.10724503841254049No Hit
CCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCAC82770.10711562482690933No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC82380.10661091184294783No Hit
TGAAGGTTCTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATT82260.10645561554019041No Hit
ACACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGA82200.10637796738881174No Hit
CCGGTGTGGCCAATGCCCTGGCTCACAAGTACCACTGAGACCCTGGCCTA81530.10551089636508296No Hit
CTGGAGAGAACCTTCGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCA81300.10521324511813131No Hit
CAGCAGCCTGCCCAGGGTCTCACCACCAACTTCGTCCACGTTCACCTTGC80850.10463088398279109No Hit
GTCAAGTCCACCTGGGGCAAGATCGGCGGCCACGCGGGCGAGTATGGCGG80780.1045402944728493No Hit
CGGGCGAGTATGGCGGAGAGGCTCTGGAGAGAACCTTCGCGTCCTTCCCC80520.10420381915020828No Hit
GCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCACGCCTACAAGCTGCGTG80240.10384146111044104No Hit
CCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTG79990.10351792714636315No Hit
GTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGAGCACC79890.10338851356073199No Hit
GCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGG79780.1032461586165377No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC79650.1030779209552172No Hit
GTCAGCACGGTGCTCACGGCGCTGAAGAACTTGTCCAGGGAGGCGTGGAC79650.1030779209552172No Hit
CGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCT79560.10296144872814918No Hit
GCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACC79210.10250850117844011No Hit
GAGAACCTTCGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCACTTCG78690.1018355505331581No Hit
CCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGC78660.10179672645746875No Hit
CAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCC78650.10178378509890564No Hit
TTTGGAGGTCAGCACGGTGCTCACGGCGCTGAAGAACTTGTCCAGGGAGG78130.10111083445362361No Hit
GGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGAGCAC77760.10063200418678833No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)