FastQCFastQC Report
Thu 20 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P1_B1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P1_B1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4600991
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCTCGGTT165040.3587053310906281TruSeq Adapter, Index 16 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCTCGGGT84700.18409077522646752TruSeq Adapter, Index 16 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCGCGGTT73630.1600307412033625TruSeq Adapter, Index 16 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCGCGGGT67460.14662058673881342TruSeq Adapter, Index 16 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCTCGTTT61000.13258013327998253TruSeq Adapter, Index 16 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)