FastQCFastQC Report
Thu 20 Jul 2023
HLTLYDMXY_n01_CIVR__Ferret_mRNA_P1_A3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTLYDMXY_n01_CIVR__Ferret_mRNA_P1_A3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13117903
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGATTCATCTCGTAT195720.14920067635810388TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGATTCATCGCGTAT133850.10203612574357351TruSeq Adapter, Index 10 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)