FastQCFastQC Report
Thu 29 Jul 2021
HLTGKBGXJ_n01_MdPS90_Md-B_t.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTGKBGXJ_n01_MdPS90_Md-B_t.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30496442
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATGCC15726275.156755663496745TruSeq Adapter, Index 7 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT417040.13675037894584555TruSeq Adapter, Index 7 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)