FastQCFastQC Report
Sat 14 Apr 2018
HLTFNBGX5_n01_pdex_gr_dam_bzip1_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFNBGX5_n01_pdex_gr_dam_bzip1_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1973060
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG80320.40708341358093525TruSeq Adapter, Index 6 (100% over 63bp)
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG69410.35178859233880366No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67680.3430204859456884No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCGACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG51590.26147202822012505No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGAGACAAGGGTTCACATTTCG49390.25032183511905365No Hit
GGTCGCGGCCGAGGATCGGTTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC46190.2341033724265861No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTGAGTGTCGCCCATGGGCATCGACACC44900.22756530465368516No Hit
GGTCGCGGCCGAGGATCCGGACTTGGAATCGTCGAGAAAAGTTTACCGGGTCCGAGGATTTGTCGACCAGGAGTG27180.13775556749414616No Hit
GGTCGCGGCCGAGGATCGATTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC22240.11271831571264938No Hit
GGTCGCGGCCGAGGATCGAGGGATGGTATCGGATATCGACACGAGGAATGACCGACCGTCCGGCCGCCGGGATTT20480.10379816123179224No Hit
GGTCGCGGCCGAGGATCGAGGGATGGTATCGGATATCGGCACGAGGAATGACCGACCGTCCGGCCGCCGGGATTT20400.10339269966448054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG451300.067.269461
GTCGCGG463350.065.547222
TCGCGGC470950.064.474793
CGCGGCC479800.063.5298924
CGGCCGA496050.061.509736
GCGGCCG498700.061.212095
GATCCAT37950.060.67932514
GGCCGAG507250.060.2126467
GGATCCA67300.058.44489713
GGATCGG19150.057.60460713
GCCGAGG539000.056.6721958
GGATCAC17950.055.69415313
AGGATCA131900.055.5379912
AGGATCT148250.055.4354512
GGATCTT59700.055.08712413
GGATCAG16450.054.90493813
GGATCTG23950.054.83975213
CCGAGGA558500.054.3725249
GATCGGT12750.054.07491314
GGATCTC37350.053.80888413
Produced by FastQC (version 0.11.2)