FastQCFastQC Report
Sat 14 Apr 2018
HLTFNBGX5_n01_pdex_gr_dam_bzip1_250_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFNBGX5_n01_pdex_gr_dam_bzip1_250_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24112724
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG1011140.41933876902501765No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCGACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG742680.3080033595540678No Hit
GGTCGCGGCCGAGGATCGGTTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC687180.2849864660666294No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGAGACAAGGGTTCACATTTCG671700.27856661901824115No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTGAGTGTCGCCCATGGGCATCGACACC625280.259315372249108No Hit
GGTCGCGGCCGAGGATCCGGACTTGGAATCGTCGAGAAAAGTTTACCGGGTCCGAGGATTTGTCGACCAGGAGTG416500.17273038085618198No Hit
GGTCGCGGCCGAGGATCGATTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC330990.1372677761334638No Hit
GGTCGCGGCCGAGGATCGAGGGATGGTATCGGATATCGACACGAGGAATGACCGACCGTCCGGCCGCCGGGATTT269880.11192431016918702No Hit
GGTCGCGGCCGAGGATCGAGGGATGGTATCGGATATCGGCACGAGGAATGACCGACCGTCCGGCCGCCGGGATTT261360.10839090597976404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG4235050.068.5130161
GTCGCGG4318850.067.168652
CGCGGCC4330950.067.136484
CGGCCGA4338650.067.020236
TCGCGGC4332800.066.944953
AGGATCT1104900.066.04043612
GCGGCCG4440850.065.4376145
GCCGAGG4449650.065.289728
AGGATCA968700.064.9371212
GGATCTA218450.064.73785413
GAGGATC4378650.064.40935511
GGATCTT442550.064.3787613
GGATCTC274700.063.6679513
GGATCGG208600.063.59682513
GGCCGAG4576200.063.527287
GGATCCA695950.062.92289713
AGGATCC1541750.062.7217412
GGATCTG210400.061.6927213
GGATCAT404700.061.68506213
GGATCAA346900.061.51782613
Produced by FastQC (version 0.11.2)