FastQCFastQC Report
Sat 14 Apr 2018
HLTFNBGX5_n01_pdex_gr_dam_bzip1_200_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFNBGX5_n01_pdex_gr_dam_bzip1_200_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29481956
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG1090800.3699890197244715No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCGACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG792910.26894755558281136No Hit
GGTCGCGGCCGAGGATCGGTTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC726880.2465508055164318No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGAGACAAGGGTTCACATTTCG722800.24516690819292994No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTGAGTGTCGCCCATGGGCATCGACACC635580.21558271099787274No Hit
GGTCGCGGCCGAGGATCCGGACTTGGAATCGTCGAGAAAAGTTTACCGGGTCCGAGGATTTGTCGACCAGGAGTG420420.14260247861437686No Hit
GGTCGCGGCCGAGGATCGATTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC352860.1196867670516841No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG5016800.068.5597841
GTCGCGG5143700.066.759432
CGCGGCC5164850.066.725944
TCGCGGC5169850.066.523683
CGGCCGA5177250.066.461226
AGGATCT1381300.065.4922812
GCGGCCG5284400.064.9690865
GCCGAGG5278200.064.9059458
AGGATCA1176050.064.3081112
GGATCTT568400.064.0871313
GGATCTA280350.063.86046613
GGATCGG226850.063.3892613
GGATCTC329450.063.2692813
GAGGATC5297950.063.07737411
GGCCGAG5450550.062.822697
GGATCCA840900.062.371313
GGATCAC165200.061.2091513
GGATCAA426150.061.00715313
GATCCAT565150.060.99680314
GGATCTG253850.060.87079213
Produced by FastQC (version 0.11.2)