FastQCFastQC Report
Sat 14 Apr 2018
HLTFNBGX5_n01_pdex_gr_dam_200_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFNBGX5_n01_pdex_gr_dam_200_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37467023
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG881730.2353349504175979No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCGACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG735560.19632197626163148No Hit
GGTCGCGGCCGAGGATCGGTTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC587410.1567805373808322No Hit
GGTCGCGGCCGAGGATCCACAAGACACTCTCCTTTTGGAAGATGACGTTTGATATCATCGAGTAGAGGGTATTTT526230.14045151118625038No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGAGACAAGGGTTCACATTTCG408280.10897049386603254No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG5573100.068.56661
GTCGCGG5700700.066.948472
TCGCGGC5720050.066.773483
CGCGGCC5739950.066.69074
CGGCCGA5756900.066.516836
GCCGAGG5801100.065.839638
AGGATCT1488100.065.7635112
GAGGATC5632850.065.4278211
AGGATCA1482750.065.3032612
GGATCTA308700.065.16540513
GGATCTT648750.064.6043813
GGATCCA911050.064.3876613
GGATCAT578300.064.04500613
GGATCGG225050.064.0032913
GGCCGAG5976100.063.909757
GGATCTC310050.063.62517513
AGGATCG861300.063.38754712
GGATCAA564400.063.15452213
GCGGCCG6070200.062.9328735
GATCCAA230350.062.57557314
Produced by FastQC (version 0.11.2)