FastQCFastQC Report
Sat 14 Apr 2018
HLTFNBGX5_n01_mdex_gr_dam_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFNBGX5_n01_mdex_gr_dam_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947875
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG69840.7368060134511407TruSeq Adapter, Index 9 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47330.4993274429645259No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGAGACAAGGGTTCACATTTCG19880.2097322959250956No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTGAGTGTCGCCCATGGGCATCGACACC16660.17576157193722802No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG14110.14885929051826455No Hit
GGTCGCGGCCGAGGAACTAACAGAACTCGCCGTGAAGACTGGCGAACAGTTCATACAGAGTCTTTTACGACTCAA11860.125121983383885No Hit
GGTCGCGGCCGAGGATCTTCTTCATACTAGTGCGGCCGCCTGCAGGGTCGACGTCCTCTCCAAATGAAATGAACT11280.11900303310035606No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCGACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG10250.10813662138995121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG209150.066.306481
GTCGCGG214700.064.606572
TCGCGGC219800.062.966293
CGCGGCC227350.061.072464
CGGCCGA236550.058.7555246
GCGGCCG239800.058.0023575
GGCCGAG242250.057.457547
GGATCCA26700.055.91504713
GGATCTC15150.054.84745413
AGGATCT65850.053.5115112
GATCCAC5000.053.0973414
GGATCGG7300.052.89897513
GATCCAT13400.052.74740614
GGATCTG10600.052.69400813
GGATCTT28450.052.35444313
AGGATCA61250.052.23889512
GGATCAT26250.052.14507313
GCCGAGG268450.051.8626828
GGATCGT6100.051.4355813
GATCATG6050.051.29076814
Produced by FastQC (version 0.11.2)