FastQCFastQC Report
Sat 14 Apr 2018
HLTFNBGX5_n01_mdex_gr_dam_bzip1_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFNBGX5_n01_mdex_gr_dam_bzip1_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863467
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG85810.9937843600276559TruSeq Adapter, Index 11 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45740.5297249344792563No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG18990.21992733943509132No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCGACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG16150.18703667887713138No Hit
GGTCGCGGCCGAGGATCGGTTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC13770.15947337883208043No Hit
GGTCGCGGCCGAGGATCCGGACTTGGAATCGTCGAGAAAAGTTTACCGGGTCCGAGGATTTGTCGACCAGGAGTG10140.1174335556541246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG185650.066.9297941
GTCGCGG191800.064.821062
TCGCGGC195200.063.5823483
CGCGGCC201150.061.8729744
GATCCAT9550.060.2734914
GCGGCCG208550.059.826315
CGGCCGA210750.059.0709236
GGCCGAG215450.057.8489077
GGATCCA22450.057.2671713
GGATCGG6100.055.3744613
GGATCAC8800.054.83509413
GTATGCC13200.054.6689545
GGATCCC7250.054.197613
CGTATGC13400.054.10752544
GATCCAA5800.054.07874714
GATCTAT3450.053.9495614
AGGATCA64000.053.5866212
AGGATCT71750.053.2268912
GGATCTC18850.053.0273413
GCCGAGG236150.052.8802648
Produced by FastQC (version 0.11.2)