FastQCFastQC Report
Sat 14 Apr 2018
HLTFNBGX5_n01_mdex_gr_dam_bzip1_250_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFNBGX5_n01_mdex_gr_dam_bzip1_250_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23270971
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG626670.2692925877480574No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCGACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG498180.21407787410331955No Hit
GGTCGCGGCCGAGGATCGGTTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC428430.18410490907319682No Hit
GGTCGCGGCCGAGGATCCGGACTTGGAATCGTCGAGAAAAGTTTACCGGGTCCGAGGATTTGTCGACCAGGAGTG308750.13267602800072245No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG274530.11797101203899056No Hit
GGTCGCGGCCGAGGATCGATTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC258300.1109966575954222No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG3681250.068.545171
CGCGGCC3800500.066.558214
GTCGCGG3796850.066.394822
CGGCCGA3806100.066.361116
TCGCGGC3802550.066.347613
GCGGCCG3834650.065.770345
AGGATCT1083900.065.7125312
GGATCCC115750.065.3763513
GGATCTT390450.065.1890413
GGATCTA213550.064.9467713
AGGATCA1084700.064.9235212
GCCGAGG3884150.064.875548
GGATCTC277400.064.3893913
GGATCCA488800.064.3095613
GATCCAT279400.064.05214
GGATCGG137850.063.77354413
GGATCAA374700.063.35639613
GGCCGAG3981800.063.2787367
GGATCAC143550.062.99444613
GGATCAT425650.062.97307613
Produced by FastQC (version 0.11.2)