FastQCFastQC Report
Sat 14 Apr 2018
HLTFNBGX5_n01_mdex_gr_dam_bzip1_200_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFNBGX5_n01_mdex_gr_dam_bzip1_200_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29732339
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG830150.2792077676768047No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCGACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG649580.2184759160723951No Hit
GGTCGCGGCCGAGGATCGGTTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC568230.1911151356104207No Hit
GGTCGCGGCCGAGGATCCGGACTTGGAATCGTCGAGAAAAGTTTACCGGGTCCGAGGATTTGTCGACCAGGAGTG418530.1407659182145071No Hit
GGTCGCGGCCGAGGATCGATTTTGTTGCCTTTTTCCGAGTTTTCTCAGCAGTTCTCGGACAAAAACTGCTGAATC343630.11557449281067325No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG303990.10224220839134117No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG5077750.068.45531
CGCGGCC5273800.066.053974
GTCGCGG5262700.065.9377752
TCGCGGC5277250.065.844283
CGGCCGA5288700.065.843856
GCGGCCG5322750.065.2450565
AGGATCT1556550.065.2045112
GGATCCC150900.065.0202513
GGATCTT561350.065.0131413
AGGATCA1510600.064.5154712
GCCGAGG5383400.064.481988
GGATCTC391500.064.141813
GGATCCA675600.063.9949113
GGATCTA315700.063.93744313
GATCCAT384500.063.677914
GGATCGG183900.063.58827613
GGATCAA520900.063.2050713
GGATCGT186750.062.91322313
GGATCAC196050.062.88305313
GGCCGAG5517950.062.8221867
Produced by FastQC (version 0.11.2)